几种理化因子对仿刺参体腔液补体溶血活性的影响  

Influence of some physical and chemical factors on the complement hemolytic activity in sea cucumber Apostichopus japonicus

作　　者：张峰[1,2] 张莉[1] 

机构地区：[1]大连水产学院生命科学与技术学院,辽宁大连116023 [2]大连水产学院辽宁省省级高校海洋生物资源可持续利用重点开放实验室,辽宁大连116023

出　　处：《大连水产学院学报》2009年第4期300-304,共5页Journal of Dalian Fisheries University

基　　金：国家自然科学基金资助项目(30471323)

摘　　要：测定了仿刺参Apostichopus japonicus体腔液补体溶血活性,并研究了几种理化因子对仿刺参体腔液补体溶血活性的影响。结果表明:仿刺参体腔液补体溶血活性为(68.8±13.5)U/mL;仿刺参体腔液在pH为7.5、Mg2+浓度为8 mmol/L的EGTA-Mg-GVB缓冲液和20℃条件下恒温水浴90 m in时,其溶血活性最高;Mg2+对仿刺参体腔液补体的溶血活性有影响,苯甲基磺酰氟(PMSF)、酵母聚糖、甲胺和肼对仿刺参补体旁路溶血活性均有明显的抑制作用。The complement hemolytic activity was reported in the coelomic fluid of sea cucumber Apostichopus japonicus, a rate of （68.8 ± 13.5） U/mL. The ACHso assay revealed that the optimum conditions for the complement hemolytic activity in the coelomic fluid were as the following- pH 7.5,8 mmol/L Mg^2＋ EGTA - Mg - GVB buffer, at 20 ℃ and reaction period of 90 min. The haemolytic activity was affected by Mg^2＋ and inhibited by zymosan, methylamine, hydrazine and PMSF.

关 键 词：仿刺参 补体溶血活性 ACH50 理化因子 

分 类 号：Q26[生物学—细胞生物学]