赖型钩体DNA重组质粒rpDJt及其表达蛋白P68对豚鼠的免疫保护研究  

Immunoprotection in Guinea Pigs Using DNA RecombinantPlasmid rpDJt and Expressed Protein P68 inL.interrogans serovar lai

作　　者：戴保民[1,2,3] 江南[1,2,3] 李胜富 方之茂[1,2,3] 赵慧业 伍卫华 叶丹霓 刘家福 杨耀 张云 刘富先 屠云人[1,2,3] 杨洪斌 黄自英 梁莉 胡利贞[1,2,3] 赵慕愚[1,2,3] 

机构地区：[1]华西医科大学 [2]成都生物制品研究所 [3]四川省卫生防疫站

出　　处：《华西医科大学学报》1998年第3期248-251,共4页Journal of West China University of Medical Sciences

基　　金：卫生部和四川省科研基金

摘　　要：为了进一步鉴定赖型钩体ＤＮＡ重组质粒ｒｐＤＪｔ及其纯化表达蛋白质Ｐ６８的免疫保护作用，采用随机、双盲和对照法对５０只豚鼠进行了３次和６个部位主动免疫接种，并于接种后３０天以强毒力、大剂量活钩体攻击。结果：Ｐ６８组存活率１００％（７／７）；ｒｐＤＪｔ组存活率７７％（１０／１３）；ｐＴ７－７组（无重组质粒）存活率２５％（３／１０）；全钩灭活疫苗组存活率９３％（１３／１４）。作者认为该质粒的表达蛋白质Ｐ６８可作为钩体基因工程亚单位疫苗的候选抗原。Immunoprotection against the infection by Leptospira interrogans serogroup Icterohemorrhagiae serovar lai strain 017 was demonstrated in guinea pigs vaccinated with DNA recombinant plasmid rpDJt and expressed protein P68 derived from genomic library of Leptospira strain 017. Thirty days after active immunization, each group received intraperitoneally (1/2 dose) and subcutaneously (1/2 dose) inoculum of L.interrogans serovar lai stain 017; cultures were adjusted to 5×108 cells/ml. All guinea pigs were observed for 10 days after challenge. Survival (%) of P68 group was 100(7/7); P23 group was 75(3/4); group rpDJt was 77(10/13); group lack recombinant (control) pT77 was 25(3/12), and group with wholecell inactivated vaccine was 93(13/14).Although the protective antigen in the leptospires has yet to be determined, it is evident that expressed protein P68 conferred a high degree of immunoprotection in guinea pigs.

关 键 词：钩端螺旋体病 重组质粒 rpDJt P68基因 

分 类 号：R514.403[医药卫生—内科学]