华佗健聪汤对Aβ所致老年痴呆大鼠海马Caspase-3活性及NGFβ含量的影响  

The Effect of Huatuo Jiancong Decoction on NGFβ and Caspase-3 Within Hippocampus of the Rats Induced Alzheimer's Disease by Aβ

作　　者：江励华[1] 段金廒[1] 王明艳[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《中华中医药学刊》2009年第9期1995-1997,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:探讨华佗健聪汤防治Aβ所致老年痴呆的作用机理。方法:22月龄SD大鼠分成正常组、假损伤组、模型组、中药预防组、中药治疗组和阳性药物对照组,中药预防组和中药治疗组分别于造模前、后给予华佗健聪汤,阳性对照组造模后给予石杉碱甲片。用凝聚态Aβ1-42定向注射各组大鼠右侧海马CA1区制备出AD动物模型,用ELISA法检测各组海马Caspase-3活性及NGFβ含量。结果:中药预防组、中药治疗组与模型组相比,NGFβ含量明显增高,而Caspase-3活性明显降低,尤其是中药预防组,数值优于正常组和阳性药物对照组。结论:本方对AD的防治作用机理与促进NGF成熟和显著的抗凋亡作用密切相关。Objective： To study the mechanism of effect of Huatuo Jiancong Decoction on preventing and curing rats with Aβ Induced Alzheimer s disease.Methods：22 months-rats were divided into in control group,model group,Chinese medicine -prevention group,Chinese medicine -treatment group and positive medicine control-group.The rat s hippocampus were injected into condensed Aβ 1-42 to make animal model.The rats of Chinese medicine-prevention group were infused intragastricly with Huatuo Jiancong Decoction beforeinjection and the rats of Chinese medicine treatment group and positive medicine control - group were infused after injection. The positive medicine was huperzmeA. Then drew the rat＇s hippocampus and and detected NGFβ and Caspase -3 by ELISA. Results ：Comparing the Chinese medicine -treatment group and Chinese medicine - prevention group to model group, NGFβ increased remarkably, however Caspase -3 decreased obviously. Especially the marks of Chinese medicine - prevention group was better than control group and positive medicine control - group. Conclusion ： The mechanism of effect of Huatuo Jiancong Decoction on preventing and curing relates closely to the function of facilitating NGF maturity and anti - Apoptosi.

关 键 词：华佗健聪汤 AΒ 阿尔茨海默病(AD) NGF βCaspase-3 抗凋亡作用 

分 类 号：R285.5[医药卫生—中药学]