花魔芋组织培养的研究  

Study on the Tissue Culture of Amorphophallus albus

作　　者：郭政宏[1,2] 乐超银[1] 王健[1] 刘海军[1] 李金鞠[1] 郭春绒[2] 

机构地区：[1]三峡大学生物技术中心,湖北宜昌443002 [2]山西农业大学文理学院,山西太谷030801

出　　处：《安徽农业科学》2009年第25期11867-11868,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30871579)

摘　　要：[目的]筛选适合花魔芋生长的诱导、分化和生根培养基。[方法]以湖北省秭归县的花魔芋球茎和鳞片作为外植体，以MS培养基为基本培养基，添加不同激素，分别组配成10种诱导培养基和分化培养基进行组织培养，然后将诱导的魔芋丛芽切成单株后接入MS＋NAA0．1—0．5mg／L生根培养基。研究不同激素配比对愈伤组织形成和芽分化以及生根的影响。[结果]球茎和鳞片在MS＋6-BA1.0mg／L＋NAA1．0mg／L培养基上容易诱导愈伤组织，诱导效率分别为92％和90％，且愈伤组织容易分化。球茎在MS＋6-BA1．5mg／L＋NAA0．15mg／L培养基上分化效率为86．7％，鳞片在MS＋6-BA2．0mg／L＋NAA0．2mg／L培养基上分化效率为83．3％。将球茎和鳞片分化出的不定芽转至MS＋NAA0．5mg／L的培养基上，生根率可达94％，20d后培养出完整植株。[结论]该试验初步建立魔芋的再生体系，为进行大规模生产魔芋种苗提供良好技术支持。[ Objective ] The study aimed to screen the media suitable to induction, differentiation and rooting of Amorphophallus albus. [Method] With the corm and scale of A. albus in Zigui County in Hubei Province as tested material, MS was used as the basic medium with different hormones to respectively match 10 kinds of media for induction, differentiation for tissue culture of A. a/bus The induced clumpy buds were cut into single plants and were put into the roofing medium of MS ＋ NAA 0.1 - 0.5 mg/L and the effects of different hormone matching on callus formation, bud differentiation and rooting were studied. [ Result] The corm and scale were easy to induce the callus on the medium of MS ＋ 1.0 mg/L 6-BA ＋ 1.0 mg/L NAA, with the induction of 92% and 90% resp. and the induced callus was easy to differentiate. The corm had the differentiation rate of 86.7% on the medium of MS ＋ 1.5 mg/L 6-BA ＋ 0.15 mg/L NAA and the scale had the differentiation rate of 83.3% on medium of MS ＋ 2.0 mg/L 6-BA ＋ 0. 2 mg/L NAA. When the adventitious buds differentiated by corm and scale were transferred into the rooting medium of MS ＋ 0. 5 mg/L NAA, the roofing reached to 94% and the whole plants were obtained after culture for 20 d. [ Conclusion] The regenerative system of A. albus was established prelim＇marily in the experiment, which provided good technique support for producing A. albus seedlings in great scope.

关 键 词：魔芋 外植体 激素 再生植株 

分 类 号：S603.6[农业科学—园艺学]