石斛兰品种遗传变异的RAPD检测  

Study on the Genetic Variation of Dendrobium by RAPD Molecular Markers

作　　者：罗远华[1] 余志金[2] 莫光武 梁定栽 莫饶[1] 

机构地区：[1]海南大学农学院,海南儋州571737 [2]海南省林业总公司,海南海口570203

出　　处：《安徽农业科学》2009年第25期11889-11891,共3页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑计划项目(2007BAD45B07)

摘　　要：[目的]利用RAPD技术检测石斛兰品种的遗传变异性。[方法]以4个母本园石斛兰品种和1个试管苗品种为材料,经DNA提取后,采用10 bp随机引物进行RAPD检测其遗传变异性。[结果]绿意(Den.Burana Green)和粉红钻石(Den.Pink Thamond)2个母本园品种未发现变异,熊猫(Den.BigPanda)和泰国白(Den.Thailand White)2个母本园品种变异率分别为3.3%和23.3%;红索尼娅(Den.Red Sonia)试管苗变异率为10.9%。[结论]建立了石斛兰品种遗传变异的RAPD检测技术。[ Objective ] The aim of research was to detect the genetic variation of Dendrobium by RAPD molecular marker. [ Method ] With 4 different varieties of Dendrobium and 1 variety in vitro as materials, after DNA extraction, 10 bp random primers was used for RAPD detection of genetic variation： [ Result ] ＂Burana Green＂ and ＂Pink Thamond＂ 2 varieties not found genetic variation; the genetic variation rate of ＂Big-Panda＂ was 3.3%, the genetic variation rate of ＂Thailand White＂ was 23.3%; the genetic variation rate of ＂Red Sonia＂ in vitro was 10.9%. [ Conservation] RAPD detection technology of genetic variation for Dendrobium was established.

关 键 词：石斛兰 品种 试管苗 RAPD 遗传变异 

分 类 号：S682.31[农业科学—观赏园艺]