镉胁迫对石竹种子萌发·叶片叶绿素及同工酶的影响  

Effect of Cadmium Stress on the Seed Germination,Leaf Chlorophyll and Isozyme of Dianthus caryophyllus

作　　者：金研铭[1] 葛冬梅 赵国禹[1] 尹秀成 于楠楠 王尊[1] 徐惠风[1] 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118 [2]长春市二道区园林管理处,吉林长春130031 [3]深圳市鲁班建设监理有限公司,广东深圳518040

出　　处：《安徽农业科学》2009年第25期11972-11973,11987,共3页Journal of Anhui Agricultural Sciences

基　　金：吉林省农业开发办公室资助项目;吉林省教育厅重点攻关项目;长春市科技局星火专家大院项目

摘　　要：[目的]研究重金属镉胁迫对石竹种子萌发、叶绿素及同工酶的影响。[方法]镉胁迫设5个处理(0、0.5、5.0、10.0、50.0mg/L),对石竹种子浸泡后测定其发芽率;对新鲜石竹浸泡后测定其叶绿素含量和同工酶。[结果]低浓度的镉对石竹的生长有促进作用,其中镉为5.0 mg/L时的发芽势比对照高15.83%;高浓度的镉抑制其生长,对根的抑制作用大于芽。随着处理时间的推移和镉浓度的增加,石竹叶绿素含量在减小,镉为5.0 mg/L处理20 d时,镉浓度与时间相关系数为-0.789;在同一处理时间内,镉浓度与叶绿素均呈显著负相关。镉胁迫下对石竹叶POD的影响主要表现为个别酶带Rf值的改变、酶带数的下降和活性的增加。[结论]重金属镉对石竹的酶活性产生影响,使其对镉胁迫产生了相对适应性。[ Objective ] The study aimed to investigate the effect of heavy metal cadmium stress on the seed germination, leaf chlorophyll and isozyme of Dianthus caryophyllus. [ Method] The cadmium stress was set up by 5 conch, treatments at 0, 0.5, 5.0,10.0, 50.0 mg/L to soak D. caryophyllus seeds and fresh leaves resp. , the seed germination energy, leaf chlorophyll and isozyme were determined. [ Result] The low concn, of cadmium had some promotion on the growth of D. caryophyllus and the germination energy in seeds treated by 5.0 mg/L cadmium was 15.83% higher than control. The high concn, of cadmium had suppression to the growth of D. caryophyUus with greater suppression to bud than to root. Along with the process of treating time and the increase of the cadmium concn., the chlorophyll content of D. caryophyllus was reduced. When D. caryophyllus leaves were treated by 5.0 mg/L cadmium for 20 d, the cadmium concn, showed a negative correlation to time, with correlation coefficient of - 0. 789. In the same treating time, the cadmium conch, showed significant negative correlation with chlorophyll content. The effects of cadmium stress on POD activity of D. caryophyllus were mainly on the change of individual enzyme belt Rf value, the drop of enzyme band number, and the increase of enzyme activeness. [ Conclusion] The heavy metal cadmium had some effect on the enzyme activeness of D. caryophyllus, which made D. caryophyllus produce the relative adaptability to cadmium stress.

关 键 词：石竹 种子萌发 叶绿素 镉胁迫 同工酶 

分 类 号：S685.99[农业科学—观赏园艺]