IGF-1经鼻给药治疗新生大鼠缺氧缺血性脑损伤  

Intranasal delivery of insulin-like growth factor-1 and its resulting in protection against hypoxic-ischemic brain damage in neonatal rats.

作　　者：马宇廷[1] 郝青英[2] 

机构地区：[1]山西医科大学,030001 [2]山西医科大学第二附属医院儿科,030001

出　　处：《中国优生与遗传杂志》2009年第9期11-12,26,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究IGF-1对缺氧缺血性脑损伤的保护性治疗作用。方法取44只7日龄Wistar新生大鼠(体重12g～16g)单纯随机分为2组,模型对照组20只,IGF-1干预组24只。模型对照组于缺氧缺血后1h鼻腔递送生理盐水0.1ml;IGF-1干预组于缺氧缺血后1h鼻腔递送IGF-1 2.5μg(溶于生理盐水0.1ml)。于缺氧缺血后24h,72 h,120h,168h断头取脑,用病理图像分析系统测大脑皮层梗死面积率和海马坏死细胞率。结果IGF-1干预组皮层梗死面积率、海马坏死细胞率,均显著低于模型对照组(P<0.05)。结论IGF-1对缺血缺氧性脑损伤具有明显的保护性作用。Objective ： To research the protective pherapeutical effect of insulin - like growth factor - 1 from hypoxic - isehemic brain damage. Methods： Forty and four 7 - day - old Wistar rats were randomly divided into two groups： IGF - 1 - treated group （with 24 rats）, and model saline control group （ with 20 rats）. IGF - 1 - treated group was delivered by intranasal with IGF - 1 2.5μg （dissolved with 0.1ml saline） after HIBD 1 hour, and model saline control group was delivered by intranasal with 0.1ml saline. All animals were killed respectively at 24hours, 72 hours, 120 hours, and 168 hours after hypoxia. The pathology image analysis sofeware was used to measure the cortex infarct square ratio and hippocampus necrosis neurons ratio. Results： The cerebral cortex infarct square ratio and hippocampus necrosis neurons ratio , IGF - 1 - treated group were all significant lower than it on model saline control group at 24hours, 72 hours, 120 hours, 168hours （ P 〈0. 05）. Conclusion：. The IGF- 1 have significant neuroprotective therapeutical effect for HIBD.

关 键 词：缺氧缺血性脑损伤 IGF-1 经鼻给药 治疗 

分 类 号：R722.1[医药卫生—儿科]