槲皮素与Cu^(2+)反应形成配合物的紫外-可见吸收光谱法研究  

Study on Complex Formation of Quercetin Reacting with Cu^(2+) Using UV-Vis Absorption Spectroscopy

作　　者：薛俊鹏[1] 李萍[1] 王齐明[1] 赵伟[1] 吴大诚[2] 

机构地区：[1]四川大学原子与分子物理研究所,四川成都610065 [2]四川大学轻纺与食品学院,四川成都610065

出　　处：《光谱学与光谱分析》2009年第9期2539-2542,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50473050)资助

摘　　要：采用带有快门的增强型瞬态光谱探测系统ICCD,实时拍摄了槲皮素在中性和酸性条件下与Cu2+反应形成配合物的紫外-可见吸收光谱。光谱每幅曝光时间为0.1 ms,Cu2+与槲皮素的摩尔比分别为0.2,0.5,1.0,2.0,5.0和10.0。实验结果表明不同摩尔比的反应物在其他条件相同时吸收光谱带的变化是相似的,但摩尔比越大反应时间越短;在中性和酸性条件下形成配合物的反应过程不一样,在中性条件下有吸收峰值为428 nm的反应中间产物出现,而在酸性条件下则直接反应生成最终产物,但它们的最终产物都只有一个吸收峰值为296 nm的吸收带;反应物暴露在空气中和隔离空气的反应过程没有差别。文章首次观测到槲皮素与Cu2+反应形成配合物有中间产物出现,且最终产物的吸收峰值为296 nm。结果为研究槲皮素-Cu2+配合物的形成机理提供了实验依据。Using an intensified spectroscopic detector. ICCD,; the real-time UV-Vis absorption spectra of quercetin （Q） reacting with Cu2＋ under neutral and acid conditions were acquired. The exposure time was 0. 1 ms for each spectrum, and the molar ratio of Cu2＋ ： Q was 0. 2, 0.5, 1.0, 2.0, 5.0 and 10. 0, respectively. Results indicate that the changes in absorption bands of reaction solutions with different ratio of Cu2＋ ： Q were similar if other conditions were the same, and the greater the molar ratio, the shorter the reaction time; the reaction processes of complex formation were different under neutral and acid conditions. There was an intermediate product with the absorption peak at 428 nm that occurred during the reaction under neutral condition, but the final product was directly formed under acid condition, and the final products under both conditions had the same absorption bands centered at 296 nm; reaction processes with air and without air showed no difference. The present work first observed that there was an intermediate product formed during the complex formation of quercetin reacting with Cu2＋ and the absorption band of the final complex was centered at 296 nm. Results obtained here provide useful experimental evidence for the study of complex mechanism of quercetin with Cu2＋.

关 键 词：槲皮素 CU2+ 配合物 ICCD 紫外-可见吸收光谱 

分 类 号：O433.5[机械工程—光学工程]