基于紫外吸收光谱的癌细胞模型研究  

The Study of Cervical Cancer Cells Model Based on UV Absorption Spectrum

作　　者：林晓钢[1] 潘英俊[1] 郭永彩[1] 张玮[1] 赵一石[1] 

机构地区：[1]重庆大学光电技术及系统教育部重点实验室,重庆400030

出　　处：《光谱学与光谱分析》2009年第9期2547-2550,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金重点项目(90510020);国家大学生创新性实验计划课题项目(CQUCX-G-2007-32);重庆市科委自然科学基金计划项目(CSTC;2008BB3159)资助

摘　　要：细胞周期是生命起源最基本也最重要的过程,是细胞全部生理过程的综合体现,文章从紫外吸收光谱随其细胞周期的变化入手,以一种简单且能准确反映癌细胞的生长变化过程的方式建模。通过人工诱导细胞同步化方法对子宫颈癌细胞进行处理,获取了处于G1期、S期、G2期和M期的同步化细胞样品,通过测量这些样品的紫外-可见光光谱,对不同周期细胞紫外-可见光光谱的吸光度随细胞周期中芳香族氨基酸、蛋白质及核酸等物质的变化规律进行了分析。根据细胞周期中不同阶段样品对应紫外-可见光光谱在204和260 nm处的吸光度和时间点的关系,利用分段线性回归法建立了子宫颈癌细胞紫外吸收光谱模型。可对细胞周期进行判断,为对细胞周期进行分析研究提供了新的依据,也为细胞建模提供了新思路。Cell cycle is the most important process in life, and embodies all the physiological processes in the cell. In order to reflect the process of cell growth simply and accurately, the authors took cell cycle into account when the Hela cell spectral model was designed. The artificially induced cell synchronization method was employed to make Hela cell in G1, S, G2 and M phases of the cell cycle. The UV absorption spectra of these Hela cell samples were measured. The absorbability of aromatic amino acid, protein and nucleic acid in different stages of the cell cycle showed the changes in cell cycle. The subsection linear regression model of cell UV absorption spectrum was designed according to the relationship between the stage and the absorbance of samples in different phase at 204 and 260 nm. The models can be used to estimate the cell cycle after experimental verification and provide a new method for analyzing cell cycle and building cell model.

关 键 词：紫外-吸收光谱 细胞模型 细胞周期 子宫颈癌细胞 

分 类 号：O434.1[机械工程—光学工程]