北京市男男性接触HIV-1感染者膜蛋白基因序列测定及亚型分析  

Env gene sequencing and HIV-1 subtyping of infected MSM from Beijing

作　　者：张洪海[1] 孙玉[1] 柳雅立[1] 吴亚松[1] 代丽丽[1] 吴昊[1] 陈德喜[1] 

机构地区：[1]首都医科大学附属北京佑安医院,100069

出　　处：《中华实验和临床病毒学杂志》2009年第4期275-277,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金面上项目（30770742）;北京市科委科技计划重大项目（D0906003040591）

摘　　要：目的了解北京市男男性接触人群（MSM）中HIV-1的最新流行趋势及膜蛋白v3环序列特征。方法巢式聚合酶链式反应（n．PCR）扩增2007年提取的北京市男男性接触HIV感染者基因组DNA样品，对膜蛋白基因c2-v3区测序，进行病毒亚型及v3环序列特点分析。结果11例样本中，4例是欧美B亚型，5例是AE重组亚型，1例是Bc重组亚型，1例是01B重组亚型。v3环顶端四肽以GPGQ和GPGR为主。结论北京市男男性接触HIV-1感染者中重组亚型呈蔓延流行趋势。Objective To comprehend the latest HIV- 1 epidemic tendency and the character of V3 loop in MSM population of Beijing. Methods The C2-V3 regions of the HIV envelop gene were amplified by nest-PCR and sequenced from 11 HIV-1-infected MSM in Beljing in 2007. The subtype and sequences of V3 loop was analyzed. Results There are 4 subtype B strains, 5 CRF AE, 1 CRFO7BC and 1 CRF15-01B strains within all 11 strains. There are five types of central motifs of the 11 samples, in which GPGR and GPGQ are most common. Conclusion Recombination subtype of HIV-1 are spread extensively in MSM population of Beijing.

关 键 词：HIV-1 性传播性疾病 病毒性 基因 

分 类 号：R512.91[医药卫生—内科学] R373.9[医药卫生—临床医学]