miRNA在HBV从感染经由肝硬化到肝癌进程中表达谱的变化  

Differential expression profile of microRNAs in the different stages of hepatitis B virus infection-related hepatocarcinogenesis

作　　者：马兆龙[1,2] 杨炼[3] 陈立波[1] 黄金明[4] 王冬冬[1] 王国斌[5] 

机构地区：[1]华中科技大学同济医学院附属协和医院肝胆外科中心,湖北省武汉市430022 [2]山东省泰安市中心医院,山东省泰安市271000 [3]华中科技大学同济医学院附属协和医院放射科,湖北省武汉市430022 [4]华中科技大学同济医学院附属协和医院消化内科,湖北省武汉市430022 [5]华中科技大学同济医学院附属协和医院腹腔镜外科治疗中心,湖北省武汉市430022

出　　处：《世界华人消化杂志》2009年第20期2112-2116,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30672067;No.30700190;教育部新世纪优秀人才支持计划基金资助项目;No.NCET-07-0318~~

摘　　要：目的:分析微小RNA(microRNA,miRNA)在HBV感染到肝硬化再到肝癌进程中表达谱的变化.方法:利用miRNA芯片技术检测人正常肝脏、乙型肝炎肝硬化、HBV相关性肝癌组织中miRNA表达谱的差异.实时定量PCR检测上述3种肝脏组织中芯片结果差异性表达的miRNA,验证芯片结果的可信性.结果:与正常肝脏组织相比,乙型肝炎肝硬化和HBV相关性肝癌组织中表达上调超过2倍的microRNA有6个,分别为:hsa-miR-602、hsa-miR-129-5p、has-miR-210、hsa-miR-671-5p、hsa-miR-30b*及hsa-miR-572;表达下调超过2倍的miRNA有8个:hsa-miR-143、hsa-miR-199a-5p、has-miR-195、hsa-miR-27a、hsa-miR-99a、hsa-miR-519e、has-miR-130a及hsa-miR-597.这些正常肝脏组织相比有差异表达的miRNA,在乙型肝炎肝硬化和HBV相关性肝癌组织中表达量无明显差别.结论:从HBV感染到肝硬化再到肝癌进程中伴有microRNA表达谱的变化,且变化主要发生在进程早期.AIM： To investigate the differential expression profile of microRNAs in the different stages of hepatitis B virus （HBV） infection-related hepatocarcinogenesis. METHODS： MicroRNA microarray was used to detect the differential expression profile of microRNAs in the liver tissue taken from healthy controls as well as patients with HBV-induced cirrhosis or HBV-related hepatocellular carcinoma. Real-time quantitative PCR was performed to verify the differential expression of candidate microRNAs obtained from microarray experiment. RESULTS： Compared with normal liver tissue, six microRNAs （hsa-miR-602, hsa-miR-129-5p, has-miR-210, hsa-miR-671-5p, hsa-miR-30b＊, and hsa-miR-572） were upregulated more than two- fold, and eight microRNAs （hsa-miR-143, hsa- miR-199a-5p, has-miR-195, hsa-miR-27a, hsa- miR-99a, hsa-miR-519e, has-miR -130a and hsa- miR-597） were downregulated in the liver tissue taken from patients with HBV-induced cirrhosis or HBV-related hepatocellular carcinoma. Though these identified microRNAs showed significant differential expression between normal liver tissue and HBV-related hepatocellular carcinoma tissue and cirrhotic tissue, they exhibited no significant differential expression between HBV-related hepatocellular carcinoma tissue and cirrhotic tissue. CONCLUSION： HBV-related hepatocarcinogenesis is associated with the change in the expression profile of microRNAs.

关 键 词：微小RNA芯片 微小RNA 乙型肝炎病毒 乙型肝炎病毒相关性肝癌 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学] R735.7