高迁移率蛋白B1与活化T细胞核因子2协同促进白细胞介素-2报告基因转录表达  

HMBG1 acts as a coactivator of NFAT2 in promoting interleukin-2 transcription

作　　者：刘辉[1,2] 姚咏明[1] 丁丽华[3] 王晓辉[3] 袁斌[3] 宋青[2] 叶棋浓[3] 黄翠芬[3] 盛志勇[1] 

机构地区：[1]解放军总医院第一附属医院烧伤研究所基础部,北京100037 [2]解放军总医院外科重症医学科,北京100853 [3]军事医学科学院生物工程研究所,北京100850

出　　处：《创伤外科杂志》2009年第5期407-411,共5页Journal of Traumatic Surgery

基　　金：国家重点基础研究发展规划项目(2005CB522602);国家自然科学基金(30801187;30672178)

摘　　要：目的探讨高迁移率蛋白B1(HMGB1)促进白细胞介素(IL)-2转录表达免疫效应的分子机制。方法构建HMGB1和活化T细胞核因子2(NFAT2)的真核表达质粒,分别单独转染以及共同转染人胚胎肾细胞系293T、人子宫颈癌细胞系Hela,同时转染IL-2报告基因,检测IL-2报告基因的表达活性。观察当HMGB1与NFAT2质粒共同转染细胞时,是否能协同促进IL-2报告基因的活性。结果在293T细胞中,HMGB1或NFAT2单独转染可提高活性倍数相加为6.8倍,而二者共转时报告基因活性与未刺激对照组相比提高了18.4倍。在Hela细胞中,二者单独转染可提高活性倍数相加为49.9倍,而二者共转时报告基因活性与未刺激对照组相比提高了117.7倍。结论HMGB1可与NFAT2可协同促进IL-2报告基因转录表达。Objective To investigate the molecular mechanism of interleukin （ IL ） -2 production facilitated by high mobility group box-1 protein（ HMGB1 ）. Methods Eukaryotic expression plasmid HMGB1 and nuclear factor of activated T cells-2 （ NFAT2 ） were transfected into 293T or Hela cells respectively or simultaneously. Reporter gene activity of IL-2 was detected and compared. Coordinate regulation between HMGB1 and NFAT2 was examined. Results Reporter gene activity of IL-2 increased by 6.8 times induced by solo-transfection of HMGB1 or NFAT2 in 293T cells,while increased by 18.4 times induced by co - transfection of HMGB1 and NFAT2 in 293T cells. On the other hand, reporter gene activity increased by 49.9 times induced by solo-transfection of HMGB1 or NFAT2 in Hela cells,while increased by 117.7 times by eo-transfection of HMGB1 and NFAT2. Conclusion HMGBI acts as a coactivator of NFAT2 in promoting IL-2 transcription.

关 键 词：高迁移率蛋白B1 活化T细胞核因子 白细胞介素-2 免疫 

分 类 号：R392[医药卫生—免疫学]