低氧对小鼠成骨细胞Cyr61/CCN1表达的影响  

Effects of hypoxia on expression of Cyr61/CCN1 in murine osteoblasts

作　　者：崔论[1] 王君[1] 齐进[1] 朱雅萍[1] 许福平[1] 魏立[1] 张凤华[1] 邓廉夫[1] 

机构地区：[1]上海交通大学医学院瑞金医院骨科上海市伤骨科研究所,上海200025

出　　处：《上海交通大学学报（医学版）》2009年第8期909-913,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30672145);上海市创新计划(07dz22008)~~

摘　　要：目的探讨低氧环境对小鼠成骨细胞富含半胱氨酸61(Cyr61/CCN1)表达的影响。方法分别在氧浓度和去铁敏诱导的低氧环境下培养小鼠成骨细胞,Real-time PCR和Western blotting于不同时间点检测成骨细胞低氧诱导因子-1α(HIF-1α)、Cyr61/CCN1和血管内皮生长因子的表达。分别敲除小鼠成骨细胞中HIF-1α和VHL基因,检测细胞Cyr61/CCN1的表达。结果两种低氧环境下,小鼠成骨细胞HIF-1α通路被激活,Cyr61/CCN1 mRNA和蛋白表达均显著增加(P<0.05或P<0.01)。HIF-1α基因敲除小鼠成骨细胞Cyr61/CCN1 mRNA和蛋白表达均显著减少,VHL基因敲除成骨细胞Cyr61/CCN1 mRNA和蛋白表达均显著增加(P<0.05或P<0.01)。结论低氧环境通过激活HIF-1α通路来上调小鼠成骨细胞Cyr61/CCN1的表达。Objective To investigate the effects of hypoxia on the expression of cysteine-rich protein 61（Cyr61/CCN1） in murine osteoblasts. Methods Murine osteoblasts were cultured under oxygen of lower concentration or treated with desferrioxamine, and Real-time PCR and Western blotting were employed to detect the expression of HIF-1α, Cyr61/CCN1 and vascular endothelial growth factor in osteoblasts at different time points. HIF-1α gene or VHL gene were knocked out in murine osteoblasts, and the expression of Cyr61/CCN1 in murine osteoblasts was detected. Results HIF-1α pathway was activated in murine osteoblasts under the condition of hypoxia, and the expression of Cyr61/CCN1 in osteoblasts was significantly increased （P 〈 0.05 or P 〈 0.01）. The expression of Cyr61/CCN1 was significantly decreased in murine osteoblasts with HIF-1α gene knockout, and that was significantly increased in osteoblasts with VHL gene knockout（P 〈 0.05 or P 〈 0.01）. Conclusion The expression of Cyr61/CCN1 in murine osteoblasts may be up-regulated by hypoxia through activation of HIF-1α pathway.

关 键 词：低氧 低氧诱导因子-1Α 成骨细胞 Cyr61/CCN1 血管内皮生长因子 

分 类 号：R68[医药卫生—骨科学]