巴戟天正丁醇提取物对乳鼠心肌细胞缺氧复氧损伤的防护作用  

Protective effect and mechanisms of the n-butanol extract of morinda officinalis How on neonatal rat cardiomyocytes by hypoxia/reoxygenation injury

作　　者：韩联合[1,2] 冯国清[2] 张贺鸣[2] 王奎鹏[2] 

机构地区：[1]郑州航空学院医院,河南郑州450015 [2]郑州大学医学院药理教研室,河南郑州450052

出　　处：《中国医院药学杂志》2009年第17期1435-1437,共3页Chinese Journal of Hospital Pharmacy

基　　金：河南省科技攻关项目(编号:0524410030)

摘　　要：目的:研究巴戟天正丁醇提物对乳鼠心肌细胞缺氧/复氧损伤(H/R)的防护作用。方法:采用培养的乳鼠心肌细胞建立缺氧/复氧损伤模型,实验分正常细胞培养组、缺氧/复氧损伤模型组、巴戟天醇提物小、中、大剂量组。观察心肌细胞形态并测定活性氧(ROS)、内皮素(ET-1)的含量变化。结果:巴戟天醇提物可明显改善缺氧/复氧损伤的心肌细胞形态及搏动程度;降低ROS、ET-1的含量。结论:巴戟天正丁醇提物对缺氧/复氧损伤的心肌细胞有防护作用;其防护作用可能与降低细胞液中ROS、ET-1的含量有关。OBJECTIVE To study the protective effect and its mechanisms of the n-butanol extract of morinda officinalis How（BEM） in cultured neonatal rat cardiomyoeytes by hypoxia/reoxygenation injury. METItODS The models of hypoxia/ reoxygenation were made with th first generation of cultured eardiomyocytes. Cultured eardiomyoeytes of neonatal rats were di vided into five groups： control group; hypoxia/reoxygenation （H/R） group; the groups of H/R ＋ low dose of BEM（0. 038 g ·L^- 1 ）, H/R ＋ medium dose of BEM（0. 11g ·L^- 1） and H/R ＋ high dose of BEM（0. 34 g ·L^- 1 ）. The morphologic photos of all groups were observed and analysis were made. The contents of cardiomyocytes ROS and ET-lwere tested after 30 min of hypoxia and 40 min of reoxygenation, RESULTS Being injured by hypoxia and reoxygenation, refracting power of the cardiomyoeytes declined,cardiomyoeytes beat became weak or stopped. The contents of ROS and ET-1 were both obviously increased in H/R group （ P〈0. 05, compared to the control group）. While compared with H/R group, the contents of ROS and ET-1 were sibnificantly decreased in BEM groups（0. 038 g ·L^- 1,0. 11 g ·L^- 1, 0. 34 g ·L^- 1 ）. CONCLUSION BEM has obviously protective effect in cultured neonatal rat cardiomocytes injured by hypoxia/reoxygenation, and its mechanism may be related to the decrease of contents of ROS,ET-1.

关 键 词：巴戟天正丁醇提取物 心肌细胞培养 缺氧/复氧损伤 活性氧 内皮素-1 

分 类 号：R285.6[医药卫生—中药学]