菠菜种子胰蛋白酶抑制剂对K-562细胞增殖的抑制及促凋亡作用研究  

Antiproliferative Effect and Induction of Apoptosis of Trypsin Inhibitor Isolated from Spinacia oleracea L. in K-562 Cells

作　　者：康庄[1] 冯倩[1] 曹厚利[1] 王作白[1] 杜林方[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《天然产物研究与开发》2009年第4期684-686,共3页Natural Product Research and Development

摘　　要：应用MTT法分析了菠菜种子胰蛋白酶抑制剂SOTI对人慢性髓原性白血病K-562细胞生长的影响。结果显示SOTI能够抑制K-562细胞的增殖,其抑制细胞增殖的作用呈现明显的剂量-效应关系;进一步观察了不同浓度的SOTI处理K-562细胞所导致的形态学变化和诱导凋亡作用,证实SOTI具有明显的体外抗癌活性,SO-TI的抗癌活性与其诱导凋亡有关。The antiproliferative effect of Spinacia oleracea L. trypsin inhibitor（SOTI） was determined by MTT assay in human chronic myelogenous leukemia K-562 cell line. The data indicated that SOTI obviously inhibited the proliferation of K-562 cells in a dose-dependent manner. SOTI also showed to cause a morphological change and to induce the phenomena of DNA Ladder in K562 cells. The results suggested that SOTI may have potential for the development of new anti-tumor agent.

关 键 词：菠菜种子 蛋白酶抑制剂 K-562细胞 抗肿瘤 细胞凋亡 

分 类 号：Q946.885[生物学—植物学] R285[医药卫生—中药学]