自组装碳纳米管-氯洁霉素分子印迹溶胶-凝胶电化学传感器研究  

Research on Self-assembly MWNTs-clindamycin Molecularly Imprinted Sol-gel Electrochemical Sensor

作　　者：胡宇芳[1] 张朝晖[1,2] 张华斌[1] 余晓晓[1] 姚守拙[2] 

机构地区：[1]吉首大学化学化工学院,吉首416000 [2]湖南大学化学传感与计量国家重点实验室,长沙410082

出　　处：《高等学校化学学报》2009年第9期1703-1708,共6页Chemical Journal of Chinese Universities

基　　金：湖南省自然科学基金(批准号:08JJ4004);湖南省教育厅优秀青年项目(批准号:07B061);化学生物传感与计量国家重点实验室开放基金;吉首大学研究生创新项目(批准号:JGY200901)资助

摘　　要：结合自组装技术,采用电聚合方法在碳纳米管修饰金电极表面制备对氯洁霉素具有特异性识别位点的分子印迹溶胶-凝胶薄膜,成功构建了一种新型印迹溶胶-凝胶电化学传感器.通过循环伏安法(CV)、示差脉冲法(DPV)、安培计时法(I-t)和扫描电镜(SEM)表征了该印迹溶胶-凝胶膜的电化学性能和表面形貌.结果表明,该传感器具有良好的选择性和灵敏度,氯洁霉素在多壁碳纳米管修饰的印迹溶胶-凝胶传感器上的响应明显提高.该印迹溶胶-凝胶传感器对氯洁霉素的浓度响应线性范围为5.0×10-7～8.0×10-5mol/L,检出限为2.44×10-8mol/L.该传感器被成功地用于人体尿液中氯洁霉素的分析测定.A novel electrochemical sol-gel imprinted sensor was developed via molecular imprinting technique, sol-gel technology and self-assembled membrane method, and thin film of molecularly imprinted sol-gel polymers with the specific binding sites for elindamycin was cast on MWNTs modified gold electrode by electropolymerization. The electrochemical performance and morphology of imprinted sol-gel film were ehareeterized by cyclic voltammetry（ CV）, differential pulse vohammetry（ DPV）, amperometric I-t curve and scanning eleetron microscope （SEM）. The results show that the sensor has good selectivity and sensitivity, and the response to elindamyein on the MWNTs modified sol-gel imprinted sensor increases clearly. The linear range is over the range from5.0×10^-7,-8.0×10^-5mol/L, and the limit of detection is 2.44 ×10^-8 mol/L. The sensor has been successfully employed to detect clindamyein in human urine.

关 键 词：分子印迹溶胶-凝胶 多壁碳纳米管 电化学传感器 电聚合 氯洁霉素 

分 类 号：O657[理学—分析化学]