动态泡沫浮选法分离富集人参提取液中的二醇型人参皂苷  

Separation of 20(S)-Protopanaxdiol Type Ginsenosides from Panax ginseng Extract by Dynamic Foam Flotation

作　　者：王玉堂[1] 刘学波[1] 岳田利[1] 刘邻渭[1] 张寒琦[2] 

机构地区：[1]西北农林科技大学食品科学与工程学院,杨凌712100 [2]吉林大学化学学院,长春130012

出　　处：《高等学校化学学报》2009年第9期1713-1716,共4页Chemical Journal of Chinese Universities

基　　金：西北农林科技大学科研启动项目(批准号:01140409)资助

摘　　要：采用动态泡沫浮选法分离富集人参提取液中的二醇型人参皂苷,用高效液相色谱法测定6种人参皂苷Rg1,Re,Rb1,Rc,Rb2和Rd的含量.考察了浮选液pH值、电解质NaCl浓度、载气流量、料液浓度及料液流速对人参皂苷浮选率的影响,确定了动态泡沫浮选的最佳条件,并与溶剂提取法、溶剂浮选法以及静态泡沫浮选法进行了比较.结果表明,动态泡沫浮选法对二醇型人参皂苷Rb1,Rc,Rb2和Rd具有高富集效率,回收率分别为93.3%,98.6%,96.9%和98.3%,而对三醇型人参皂苷Rg1和Re的富集效率却很低,回收率分别为4.8%和4.2%.该法是分离纯化二醇型人参皂苷的一种简便有效的方法.In this study dynamic＇foam flotation was applied for the separation and enrichment of 20 （S）-protopanaxdiol type ginsenosides in Panax ginseng extract and high performance liquid chromatograph（HPLC） was applied to determine ginsenoside Rgl, Re, Rb1 , Rc, Rb2 and Rd. The effects of pH of the solution, concentration of electrolyte NaCl, the flow rate of carrier gas, initial concentration and the flow rate of the extract on the dynamic foam flotation were investigated. The optimal condition was selected. Dynamic foam flotation was compared with solvent extraction, solvent flotation and foam flotation. The experimental results show that the enrichment efficiency of20（S）-protopanaxdiol type ginsenoside Rb1, Rc, Rb2 and Rd by dynamic foam flotation is the highest, the recoveries are 93. 3%, 98. 6%, 96. 9% and 98. 3%, respectively. But the enrichment efficiency of 20 （S）-protopanaxtriol type ginsenoside Rg1 and Re is very low, the recoveries are 4.8% and 4.2%, respectively. This method for separation and purification of 20（S）-protopanaxdiol type ginsenosides is convenient and effective.

关 键 词：泡沫浮选法 人参 人参皂苷 高效液相色谱法 

分 类 号：O652[理学—分析化学]