高效液相色谱法分析丁硫克百威及其降解产物  

Quantitative Analysis of Carbosulfan and Its Degradation Product by HPLC

作　　者：侯宪文[1] 李勤奋[1] 邓晓[1] 李光义[1] 黄梁财[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《农药》2009年第9期654-655,657,共3页Agrochemicals

基　　金：海南省自然科学基金(808192);中国热带农业科学院环植所博士启动项目(Hzs0804)

摘　　要：建立丁硫克百威及其降解产物(克百威、3-羟基克百威)的高效液相色谱分析方法。液相色谱条件:流动相为甲醇-水(体积比50:50),流速1.0mL/min,C18柱,紫外检测器波长269nm,进样体积10.0μL。利用峰高进行外标法定量分析,线性相关系数大于0.999,检出限5.5～18.2μg/L。丁硫克百威、克百威和3-羟基克百威峰高值的变异系数分别为0.45%、0.86%、1.11%,回收率分别为102.4%～103.3%、106.9%～108.4%、102.6%～104.7%。丁硫克百威及其降解产物可同时检测和定量分析。A method for quantitative analysis of carbosulfan and its degradation product （carbofuran, 3-hydroxy carbofuran） by HPLC analysis was established. Liquid chromatography conditions： mobile phase was methanol- water（50：50, by vol） at a flow rate of 1.0 ml/min; c18 column; UV detector wavelength： 269 nm; injection volume： 10.0 μL. Use of external standard peak height for the quantitative analysis, linear correlation coefficient greater than 0.999, and the detection limit 5.5-18.2 μg/l. the variation coefficient of peak value for carbosulfan,carbofuran and 3-hydroxy carbofuran were 0.45, 0.86 and 1.11%, separately. The reclaim ratios were between 102.4-103.3%, 106.9-108.4%, 102.6-104.7%. Results indicate the carbosulfan and its degradation products at the same time detection and quantitative analysis.

关 键 词：丁硫克百威 降解产物 高效液相色谱 定量分析 

分 类 号：TQ450.7[化学工程—农药化工]