重楼对多发骨折—脂多糖两次打击模型大鼠的保护作用  

Protective Effects of Rhizoma Paridis Total Saponins on the Rats Subjected to Two-hit Induced by Multiple Fractures and Lipopolysaccharide.

作　　者：刘祥[1] 马秀[1] 彭月航[1] 吴开莉[1] 韩毅[1] 周满红[1] 

机构地区：[1]遵义医学院第一附属医院急诊科,贵州遵义563003

出　　处：《四川中医》2009年第9期14-17,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：贵州省科学技术基金资助(编号:黔科合J[2005]2075)

摘　　要：目的:探讨重楼总皂苷(RPTS)对多发骨折—脂多糖两次打击模型大鼠的保护作用。方法:将70只Wistar大鼠随机分成3组,除空白对照组外,其余2组按多发骨折—脂多糖两次打击致脓毒症并急性肺损伤动物模型标准制模,制模后1小时,重楼干预组予重楼总皂苷(RPTS)灌胃。干预后6小时,部分动物采血,以ELISA法检测血清中TNF-α、IL-1β及IL-6浓度;各组留取肺组织,光镜下进行病理形态观察。剩余动物用以观察死亡率。结果:重楼干预组大鼠血清TNF-α、IL-1β及IL-6浓度显著低于模型对照组(P<0.001)。死亡率,重楼干预组显著低于模型对照组(P<0.05)。结论:重楼总皂苷对多发骨折-脂多糖两次打击模型大鼠具有保护作用,机制可能与下调血清中的TNF-α、ΙΛ-1β、IL-6水平从而减轻炎症反应以及减轻急性肺损伤有关。Objective： To investigate the. protective effects of Rhizoma Paridis total, saponins （BPTS） on the rats subjected to two - bit induced by multiple fractures and lipspolysaccharide. Methods ： Eighty six Wistar rats were randomly divided into three groups, and were made to the model in accordance with the standard of two - hit animal model induced by multiple fractures and lipopolysaccharide, except the blank control group. 1 hour later, rats in RPTS groups were given Rhizoma Paridjs total saponins by intragastric administration.6 hours later, the concentrations of TNF-α, IL-1β and IL- 6 in the blood serum of part rats were detected by ELISA （enzyme linked immunosorbent assay） . The pathological changes of the lung tissues were examined with light nficroscope.The mortality rate were estimated from another part rats. Results ： In RPTS groups, the concentrations of TNF-α,IL- 1β and IL - 6 in blood serum of rats were remarkably lower than model group （ P 〈 0.001 ） .In the model group, the evidence of lung injury was discovered by light microseopy. In RPTS groups, the histological changes of lung injury were ameliorated. The mortality rate in RPTS groups was lower than the model. Conclusion： Rhizoma Paridis total saponins can protect the rats subjected to two-hit induced by multiple fractures and lipopolysacchuride by falling the concentrations of TNF-α, IL-1β and IL-6 in blood serum of rats and inhibiting acute lung injury

关 键 词：重楼总皂苷 多发骨折 脂多糖两次打氨急性肺损伤 死亡率 

分 类 号：R285.5[医药卫生—中药学]