水稻新型MT基因的鉴定及其基因家族分子特征分析  

Identification of A Novel Type of MT Gene in Rice and Analysis on the Molecular Characteristics of Its Gene Family

作　　者：徐玉凤[1] 李一勤[1] 刘进元[1] 

机构地区：[1]清华大学生命科学与技术系分子生物学与蛋白质科学实验室,北京100084

出　　处：《安徽农业科学》2009年第26期12440-12442,12461,共4页Journal of Anhui Agricultural Sciences

基　　金：国家重点基础研究发展计划(2006CB101706);国家自然科学基金(30270753;30370804)资助项目

摘　　要：[目的]探讨水稻MT基因家族各成员在基因结构上的差异及相互间的进化关系。[方法]从经重金属处理的水稻幼苗中分离到3个新的植物MT,对其进行RT-PCR和Northern杂交分析以及水稻和拟南芥MT基因家族的序列比对和进化分析。[结果]RT-PCR和Northern杂交分析表明,水稻中3个MT基因的表达均受重金属的诱导,在幼苗根部进行特异性表达。由水稻和拟南芥MT基因家族的序列比对提出植物MT的新分类方式。水稻I类OsMT基因家族的所有10个成员,定位到6条染色体上。同一类型的OsMT有高度的氨基酸序列保守性,相似的Cys排列方式。新发现的3个OsMT家族在单子叶植物水稻中独有,属4型MT。[结论]水稻和拟南芥MT基因家族进化分析显示,植物MT至少发生在种子植物出现前,而4型MT可能发生于单子叶植物和双子叶植物分离后。[ Objective ] This study aimed to explore the difference of each members of metallothionein （Mr） gene family on gene construction and the evolution relationship among them each other. [ Method] 3 novel plant MT genes in rice seedlings treated by heavy metal were isolated and they were made for RT-PCR and Northern blot analysis and the sequences alignment and evolution analysis of MT gene family between rice and Arabidopsis thaliana. [ Result] RT-PCR and Northern blot analysis showed that in rice seedlings, the 3 MT genes were all induced by heavy metals and were dominantly expressed in roots. The new classification mode for plant MT was proposed on based of the sequences align- ment of MT gene family between rice and A. thaliana. All 10 members of rice class I OsMT family were unevenly distributed in 6 chromo- somes. The same type of OsMT showed a high conservation for amino acid sequence and the similar Cys-arrangement pattern. The novel 30s- MT families were only particularly found in the monocotyledon of rice and were classified to type 4 of MT. [ Conclusion ] The evolution analysis on MT gene family of rice and A. thaliana showed that the plant MT gene dated back at least to the origin of seed plants and type 4 of MT might originate after the monocotyledon-eudicot divergence.

关 键 词：金属硫蛋白 水稻 NORTHERN杂交 基因表达 

分 类 号：S511[农业科学—作物学]