戊二醛熏蒸和浸泡对内镜细胞刷灭菌效果的研究  

Efficiency of glutaraldehyde immersion and fumigation on sterilization of endoscopic cell brushes

作　　者：黄瑞娟[1] 魏明[1] 李线明[1] 李月玲[1] 李党育[1] 赵红斌[1] 

机构地区：[1]广西壮族自治区南溪山医院感染管理科,广西桂林541002

出　　处：《中国内镜杂志》2009年第8期867-869,共3页China Journal of Endoscopy

摘　　要：目的比较戊二醛熏蒸和浸泡对内镜细胞刷的灭菌效果,探讨戊二醛熏蒸灭菌的可行性。方法内镜细胞刷浸染枯草杆菌黑色变种芽孢(ATCC9372),干燥后分别经戊二醛浸泡3、5和10h和戊二醛熏蒸3、5h灭菌处理,采样进行培养。结果内镜细胞刷经戊二醛浸泡3h,不能完全杀灭细胞刷污染的枯草杆菌黑色变种芽孢;浸泡5和10h则可完全杀灭芽孢,达到灭菌合格;经戊二醛熏蒸3和5h,不能完全杀灭细胞刷污染的枯草杆菌黑色变种芽孢,达不到灭菌合格。结论常压下戊二醛气体熏蒸不适用于内镜细胞刷灭菌,戊二醛浸泡5和10h可达到灭菌合格。[ Objective ] To explore the sterilized efficiency of glutaraldehyde immersion and fumigation for endoscopic cell brush. [Methods] The endoscopic cell brushes contaminated with Bacillus var.niger spore（ATCC9372） were sterilized by dipping in glutaraldehyde for 3, 5 and 10 h, and fumigation with glutaraldehyde for 3 and 5 h. Then the brushes were sampled for germiculture. [ Results ] The sterilization methods of dipping in glutaraldehyde for 5 h and 10 h can kill Bacillus vat.niger spore contaminated on cell brushes, but the methods of dipping in glutaraldehyde for 3 h and fumigation with glutaraldehyde for 3 h and 5 h cannot do. [ Conclusions ] The method of fumigation with glutaraldehyde at normal pressure cannot be applied to sterilization of endoscopic cell brushes. The method oft dipping in glutaraldehyde for 5 h and 10 h can be used in sterilization of cell brushes.

关 键 词：内镜细胞刷 戊二醛 熏蒸 浸泡 

分 类 号：R613[医药卫生—外科学]