后纵韧带骨化患者脊柱韧带细胞表型分析  

Phenotype of spinal ligament cells from patients with ossification of posterior longitudinal ligament

作　　者：颜廷宾[1] 王修文[2] 张佐伦[3] 林琳[4] 

机构地区：[1]山东大学齐鲁医院骨创科,山东济南250012 [2]山东大学第二医院骨科 [3]山东省立医院脊柱外科 [4]山东省疾病预防控制中心

出　　处：《中国公共卫生》2009年第9期1098-1100,共3页Chinese Journal of Public Health

基　　金：山东省科技攻关计划资助项目(2007GG30002019)

摘　　要：目的检测后纵韧带骨化(OPLL)患者脊柱韧带细胞体外培养的生物学特性。方法分离并体外培养后纵韧带骨化患者非骨化部位和非后纵韧带骨化患者的脊柱韧带组织,建立8个骨化细胞系(OL1-OL8)和7个非骨化细胞系(NL1-NL7),观察其体外培养条件下的细胞表型。结果8个OL细胞系中,有4个OL细胞系表达成骨细胞表型,具有较高的碱性磷酸酶(ALP)活性[(1.05±0.43)U/mL],可形成骨小结节,阿尔新兰及茜素红染色呈阳性,可分泌骨钙素(OC)(OL1:3.7 ng/mL,OL3:3.5 ng/mL,OL7:2.9 ng/mL,OL8:2.6 ng/mL)。而7个NL细胞系的ALP活性均低(0.47±0.05)u/mL),未测到OC分泌,不形成骨小结节,钙盐染色阴性,表现典型的成纤维细胞表型。结论成功建立脊柱韧带细胞系;后纵韧带骨化患者脊柱韧带细胞体外培养可表达成骨细胞表型。Objective To study the biological characteristic of the cultured ligament cells derived from patients with ossification of posterior longitudinal ligament of the spine （OPLL）. Methods The ligamental tissue of the spine was separated from OPLL patients and non-OPLL patients. Eight ossified cell lines （ OL1-OL8 ） and seven non-ossified cell lines （NL1-NL7） were built. The phenotype of all the cell lines were observed. Results Four of eight OL cell lines expressed osteoblast phenotype with high alkaline phosphatase（ALP） activity（ 1.05 ± 0.43 μ/mL）. The forming of bony tubercle, secretion of glycosaminoglycans, calcium deposit were observed and osteocalcinl （OC） was detected（ OL1 ： 3.7 ng/mL, OL3 ： 3.5 ng/mL, OL7：2.9 ng/mL, OL8 ： 2.6 ng/mL）. However, all NL cell lines expressed fibroblast phenotype with low ALP activity（0.47 ± 0.05 p/mL） and no bone tubercle formation and OC secretion. Conclusion Spinal ligament cell line was established successfully. Cultured ligament cells from OPLL patients can express osteoblast plienotype in vitro.

关 键 词：后纵韧带骨化 成纤维细胞 成骨细胞 表型 骨钙素 

分 类 号：R681.5[医药卫生—骨科学]