海南省HIV-1基因亚型分析  

HIV-1 gene subtypes in HIV positives and AIDS patients in Hainan province

作　　者：符鹏[1] 邓巍[2,3] 鲍琳琳[2,3] Nicole Vidal 何启亚[1] 秦川[2,3] Martine Peeters Eric Delaporte Jean-Marie Andrieu 王召乾[1] 邝继深[1] 张帆[1] 胡玉銮[1] 卢葳[3,4] 

机构地区：[1]海南省疾病预防控制中心,海口570203 [2]北京协和医学院中国医学科学院比较医学中心 [3]北京协和医学院中国医学科学院中法合作艾滋病研究中心 [4]法国发展研究院蒙特利尔大学UMR145研究所 [5]巴黎第五大学肿瘤疫苗免疫治疗中心病毒分子研究所

出　　处：《中国公共卫生》2009年第9期1142-1143,共2页Chinese Journal of Public Health

基　　金：海南省自然科学基金(807101)

摘　　要：目的分析海南省人类免疫缺陷病毒-1(HIV-1)基因亚型,监测HIV毒株的流行状况。方法从88份HIV-1抗体阳性的血浆中提取RNA,逆转录后采用PCR法扩增HIV-1基因序列中的env及pol基因,对扩增产物进行核苷酸序列测定,经比对分析而确定基因亚型。构建最大似然(ML)进化树,分析感染者间的传播链。结果88份样品中,有83份样品被扩增出HIV-1的env及pol基因片段;共发现6种HIV-1亚型和重组型,其中CRF01-AE亚型70份,B′亚型8份,C亚型2份,B亚型和CRF08-BC、CRF01-AE/B′重组亚型各1份。在ML进化树上83份样品分成4个传播群(66/83)和非传播群(17/83),其中传播群1最庞大(59/83)。结论海南省HIV/AIDS的HIV-1基因亚型至少有6种,其中CRF01-AE为主要亚型,占84.33%(70/83),主要分布在静脉吸毒者及其性伴侣之间,形成了一个庞大的传播群。其次为B′亚型(9.6%)。另外的4种亚型(6%)仅见单个病例。提示海南省静脉吸毒为主要的感染途径(67.5%);经性接触传播的亚型种类复杂多样,包括本次检出的全部亚型和重组株。Objective To monitor the epidemic situation of HIV strains in Hainan province by analyzing HIV-1 gene subtypes of HIV positives and AIDS patients. Methods PCR method was used to amplify env and pol genes of HIV-1 gene sequences after RNA reverse transcription extracted from 88 HIV-1 positive plasma samples. The nucleotide sequence of amplification products was determined, and the genetic subtype was identified by comparative analysis. The maximum likelihood（ML） phylogenetic tree was constructed for analysis of the spread chain among HIV positives. Results Env and pol gene fragments of HIV-1 were successfully amplified for 83 sample, and 6 kinds of HIV-1 subtypes and recombinant gene were found,including 70 CRF01-AE subtype samples,8 B ＇subtype samples,2 C subtype samples, 1 B subtype sample, 1 CRF08-BC sample,and 1 recombinant subtype of CRF01-AE / B＇ samples. In the ML phylogenetic tree,83 samples were divided into four disseminative groups（ 66/83 ） and non-disseminative groups（ 17/83 ）, in which the disseminative group 1 （59/83） was the largest. Conclusion There are at least 6 kinds of HIV-1 gene subtypes in HIV / AIDS patients in Hainan province, and CRF01-AE subtype accounted for 84. 33% （70/83） of all positives, mainly distributed among the intravenous drug users and their sexual partners in a large disseminative group. Next is B ＇subtype（9. 6% ）. Four other subtypes（6% ） are observed only in individual case. The results suggest intravenous drug is the main route of infection（67.5% ） in Hainan province.

关 键 词：人类免疫缺陷病毒 亚型 序列分析 进化树 

分 类 号：R373[医药卫生—病原生物学]