再生障碍性贫血外周血CD5^+B细胞检测及其临床意义分析  

The change of CD5^+B lymphocytes in the peripheral blood of patients with childhood aplastic anemia and its clinical significance

作　　者：余慧[1] 白燕[1] 邱奕宁[1] 刘勤[1] 肖燕[1] 周东风[1] 金润铭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院儿科,武汉4300022

出　　处：《中国实用儿科杂志》2009年第9期711-713,共3页Chinese Journal of Practical Pediatrics

摘　　要：目的检测再生障碍性贫血(AA)患儿外周血CD5+B细胞比例,探讨其临床意义。方法2006年1月至2008年3月华中科技大学同济医学院附属协和医院利用流式细胞术对56例AA患儿进行外周血CD5+B细胞检测,与对照组(同期体检的健康儿童60名)进行对比;同时与使用大剂量丙种球蛋白(HDIG)联合环孢素A(CSA)和泼尼松的免疫抑制治疗疗效进行相关分析。结果重型AA(SAA)患者外周血CD5+B细胞比例明显高于正常对照(P<0.01);治疗有效组CD5+B细胞比例高于治疗无效组及对照组(P<0.01),并且在治疗半年后复查呈下降趋势(P<0.05)。结论SAA患儿外周血CD5+B细胞比例增高,可能与SAA的发病有关。采用大剂量丙种球蛋白联合CSA和泼尼松治疗后不同疗效组的CD5+B细胞比例存在差异,提示可能将CD5+B细胞水平作为指导AA临床选择治疗方法的实验室指标。Objective To investigate the quantitative change of CD5^＋B lymphocytes in the peripheral blood of patients with childhood aplastie anemia and its clinical significance. Methods Quantities of CD5^＋B lymphocytes in the peripheral blood of 56 patients with childhood aplastic anemia and 60 normal controls were assayed by flow cytometry. The correlation between the therapeutic efficacy of the combined immunotherapy （HDIG＋CsA＋Prodnisone） and the quantities of CD5^＋B lymphocytes was analyzed. Results The quantity of CD5^＋B lymphocytes was significantly higher in SAA patients than in normal control; the quantity of CD5^＋B lymphocytes was significantly higher in effectively cured patients than in ineffective cured patients, and decreased after six months of treatment in effectively cured patients. Conclusion CD5^＋B lymphocytes in the peripheral blood of patients with childhood SAA increase significantly, which may be related to the onset of the disease. The quantities of CD5^＋B lymphocytes differ in groups which had different curative effect after being treated by HDIG＋CsA＋Prednisone, which suggests that CD5^＋B lymphocytes might be a useful marker to direct the clinical therapy.

关 键 词：再生障碍性贫血 儿童 CD5^+B细胞 

分 类 号：R72[医药卫生—儿科]