RP-HPLC测定光果甘草中光甘草定的含量  

Determination of Glabridin in Glycyrrhiza glabra L. by RP-HPLC

作　　者：骆从艳[1] 王新春[2] 江发寿[3] 王园姬[3] 陈文[3] 

机构地区：[1]石河子大学药学院 [2]石河子大学医学院第一附属医院 [3]新疆特种植物药资源教育部重点实验室,新疆石河子832000

出　　处：《农垦医学》2009年第4期311-313,共3页Journal of Nongken Medicine

摘　　要：目的:建立测定光果甘草中光甘草定含量的HPLC方法。方法:采用Kromasil C_(18)柱(4.6mm×250mm, 5μm),流动相为乙腈2%冰醋酸溶液(pH3)(53;47),检测波长280nm,流速1.0 mL/min,进样体积10μL。结果:光甘草定在0.0810～0.7290μg范围内呈良好的线性关系,平均回收率(n=9)为99.8%,其相对标准偏差为2.2%。结论:该方法简单、结果准确,重现性好,适用于光甘草定的含量测定。To develop an HPLC method for the determination of Glabridin existed in Herb Glycyrrhiza glabra L. The separation was performed on a reversed-phase C18 column （4.6mm×250mm,5μm） , with mobile phase of acetonitrile and 2% water-glacial acetic acid （pH3） （V ：V =53：47）. The assay was carried out at a flow rate of 1.0 mL · min-1 at room temperature, glabridin can be determined at 280nm, the sample injection was 10μL. The linear range of glabradin was 0. 0810-0.7290μg and in this range it can present a favorable linear. The average recoveries （n =9） of glabridin was 99. 8% , RSD was 2.2%. The method is simple, accurate and reproducible. It can be applied for the determination of glabridin in Glycyrrhiza glabra L.

关 键 词：RP-HPLC 光甘草定 含量测定 

分 类 号：R927.2[医药卫生—药学]