复合氨基酸注射液(17S)中N-乙酰-L-半胱氨酸的含量测定  

HPLC DETERMINATION OF N-ACETYL-L-CYSTEINE IN COMPOUND AMINO ACID INJECTION(17S)

作　　者：高春[1] 周淑平[1] 纪宏[1] 高恒莹[1] 

机构地区：[1]北京市药品检验所

出　　处：《中国医药工业杂志》1998年第9期410-412,共3页Chinese Journal of Pharmaceuticals

摘　　要：对复合氨基酸注射液（１７Ｓ）中含量较低的氨基酸Ｎ－乙酰－Ｌ－半胱氨酸用ＨＰＬＣ法进行了定量研究。结果表明，浓度在１０～１００μｇ／ｍｌ呈良好的线性关系，ｒ＝０．９９９９，ＲＳＤ＝０．９８％，平均回收率为９９．４７％。The determination of LacetylLcysteine in compound amino acid injection (17S) was studied by HPLC in the condition of chromatographic mobile phase of 0.5 mol/L 0.15 ml H2SO4 in 2000 ml water, UV detection at 210 nm, flow rate 1.0 ml/min, injection volume 20 μl and external standard. The results showed the standard curve was linear in the concentration range of 10～100 g/ml(r=0.9999). The average recovery was 99.4%(RSD=0.98%).

关 键 词：半胱氨酸 高效液相色谱 氨基酸注射液 含量测定 

分 类 号：R977.4[医药卫生—药品] R927.2[医药卫生—药学]