铜绿微囊藻毒素的肝细胞毒性及活性氧生成作用  

Hepatotoxicity and Formation of Reactive Oxygen Species Induced by Microcystis Aeruginosa Toxin

作　　者：丁文兴[1] 沈汉明[1] 朱惠刚[1] 

机构地区：[1]上海医科大学环境卫生教研室

出　　处：《中华预防医学杂志》1998年第5期278-280,共3页Chinese Journal of Preventive Medicine

摘　　要：目的探讨微囊藻毒素的肝细胞毒性及其预防措施。方法利用原代培养的大鼠肝细胞，对水华提取物微囊藻毒素进行了细胞毒性和活性氧类（ＲＯＳ）生成的研究。结果在微囊藻毒素处理的肝细胞中，可以引起明显的依赖时间和剂量的乳酸脱氢酶（ＬＤＨ）释放率的升高，同时还可以引起明显的时间依赖的ＲＯＳ的升高。在去铁敏（ＤＦＯ）的作用下，可以分别使微囊藻毒素处理的肝细胞的ＬＤＨ释放率和ＲＯＳ升成率降低。结论微囊藻毒素具有明显的肝细胞毒性。Objective To study hepatotoxicity of Microsystis aeruginosa toxin (microcystin) and its prevention. Methods Cytotoxicity and the formation of reactive oxygen species (ROS) of microcystin extracted water bloom were studied in rat liver cells of primary culture. Results It was found that the release of lactic dehydrogenase (LDH) increased with a time and dose dependent pattern in the liver cells treated with microcystin, and a time dependent formation of ROS also increased simultaneously. The antioxidant, desferrioxamine, could decrease the release of LDH and the formation of ROS in the liver cells treated with microcystin. Conclusion Microcystin has obvious hepatotoxicity and the formation of ROS may play a certain role in its toxic effect on the rat liver cells.

关 键 词：藻类毒素 铜绿微囊藻毒素 肝细胞毒性 活性氧 

分 类 号：R996.2[医药卫生—毒理学]