血糖控制对新诊断2型糖尿病患者血单核细胞核因子κB活性及血清炎症指标的影响  

The effects of glycemic control on levels of nuclear factor κB activity in peripheral blood mononuclear cell and on serum inflammation markers in newly diagnosed type 2 diabetics

作　　者：石秀林[1,2] 徐明彤[1] 李芳萍[1] 黎峰[1] 严励[1] 李焱[1] 程桦[1] 

机构地区：[1]中山大学附属第二医院内分泌科,510120 [2]福建医科大学附属厦门第一医院内分泌科

出　　处：《中国糖尿病杂志》2009年第8期619-621,共3页Chinese Journal of Diabetes

摘　　要：目的探讨血糖控制对新诊断T2DM患者外周血单核细胞(PBMNC)中核因子κB(NF-κB)活性及血清炎症指标的影响。方法 96例新诊断T2DM患者,观察治疗前及血糖良好控制后PBMNC中磷酸化NF-κBp65(Ser^(536))、血清炎症指标及FFA等变化。结果在血糖控制达标2周时,PBMNC磷酸化NF-κBp65(Ser_(536))、FFA及血清3项炎症指标水平比治疗前显著降低。△hsC-RP与A2hPG、△糖化血清蛋白(FA)呈正相关;与△HDL-C呈负相关;△IL-6与△TC、△LDL-C呈正相关(P<0.05或P<0.01)。结论新诊断T2DM患者在严格控制血糖后,血清hsC-RP、IL-6和TNF-α水平以及PBMNC中NF-κB磷酸化p65(Sers^(536))水平均明显降低。血糖改善带来炎症状态的改善。Objective To investigate the changes of levels of NF-κB activity in peripheral blood mononuclear cell （PBMNC） and of serum inflammation markers including hs-CRP, TNF-α and IL-6 after glycemic control in newly diagnosed type 2 diabetes （T2DM）. Methods 96 newly diagnosed type 2 diabetes patients were selected. Inflammation makers were assayed at 0 weeks and 2 weeks after glycemic control. Serum hs-CRP, TNF-α, IL-6 were assayed by ELISA. Phosphorylation status of NF-κB p65 in PBMNC was determined by immunoblotting method with phosphor-specific antibodies to NF-κB p65 （Sers36）. Results Significant decreases in the levels of phosphorylation NF-κB P65（Sers36） and of serum hs-CRP, TNF-α, IL-6 and FFA were found at 2 weeks after glycemic control. △hs-CRP was positively correlated with △2hPG and △FA significantly（P〈0.05 -0.01）, hut negatively correlated with △HDL-C significantly （r=-0. 305,P=0. 016）. △IL-6 was positively correlated with △TC and △LDL-C significantly （P 〈 0. 05-0. 01）. Conclusions The levels of serum hs-CRP, IL-6 and TNF-α and the level of phosphorylation NF-κB P65 are decreased at 2 weeks after glycemic control. The improvement of serum glucose contributes to the improvement of inflammation states.

关 键 词：糖尿病 2型 炎症 核因子ΚB 高敏C反应蛋白 

分 类 号：R587.1[医药卫生—内分泌] R733.7[医药卫生—内科学]