PTA1在人巨核母细胞系HEL细胞和血小板的表达及分布  

Expression and localization of PTA1 molecule on human platelets and HEL cell line

作　　者：孙忱[1,2] 金伯泉[1,2] 孙凯 李家增[1,2] 武怀珠 刘雪松[1,2] 杨琨 董邦权[1,2] 

机构地区：[1]第四军医大学免疫学教研室 [2]中国医学科学院血液学研究所

出　　处：《细胞与分子免疫学杂志》1998年第3期195-197,207,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金

摘　　要：应用抗人血小板和Ｔ细胞活化抗原１（ＰＴＡ１）单克隆抗体（ｍＡｂ）间接免疫荧光染色和流式细胞仪（ＦＣＭ）分析，以及胶体金免疫电镜技术，检测了人巨核母细胞系ＨＥＬ细胞和血小板膜表面ＰＴＡ１分子的表达水平，并对静止与活化血小板膜表面ＰＴＡ１分子的表达和分布进行了比较。结果表明，ＨＥＬ细胞与人血小板膜表面均表达高水平ＰＴＡ１分子，活化血小板膜表面ＰＴＡ１分子表达水平有所降低，但胞膜内陷所形成的胞浆空泡膜内侧可见密度较高的ＰＴＡ１分子。We investigated the expression and localization of PTA1 molecule on human megakaryocyte cell line(HEL) and resting or activated platelets by FCM analysis and immunoelectron microscopic localization. Ninetynine percent of HEL cells expressed PTA1 molecule. The expression of PTA1 molecule on activated platelets (92%) was fairly lower than that on resting platelets (97%). Compared with activated platelets, PTA1 molecule was expressed only on cell membrane of resting platelets. But in the case of activated platelets, PTA1 molecule could be found on cell membrane as well as bubbles formed from cell membrane in cytoplasm, and pseudopodia of activated platelets expressed a high level of PTA1 molecules. These findings support the hypothesis that PTA1 molecule may play important role in platelet activation and involve in human megakaryocyte differentiation and platelet maturation.

关 键 词：PTA1 巨核细胞 HEL细胞 血小板 单克隆抗体 

分 类 号：R392.11[医药卫生—免疫学]