白介素对脐血B细胞免疫球蛋白类别转换的影响  

Cytokine Regulation of ImmunoglobuUn Class Switching in Neonatal B Cell

作　　者：李庆生[1] 吴圣楣[1] 应大明[1] 林梓[1] 

机构地区：[1]上海第二医科大学附属新华医院

出　　处：《上海第二医科大学学报》1998年第5期357-359,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金资助课题(39600158)

摘　　要：采用反向酶联免疫斑点法观察脐血单个核细胞,及添加重组细胞因子后脐血 B 细胞免疫球蛋白释放细胞数量(IgSCs),以探究细胞因子对新生儿 B 细胞免疫球蛋白类别转换的影响。结果:正常脐血单个核细胞仅产生少量的 IgSCs。用抗 CD3单抗刺激丝裂霉素 C 处理后的脐血 T 细胞,并补充 rIL-2、rIL-4、rIL-10及其组合,可诱导脐血 B 细胞释放 IgA、IgG 及 IgM。表明:细胞因子的补充可促进体外新生儿 B 细胞免疫球蛋白的类别转换。To determine the probable regulatory effects of cytokines on immunoglobulin class switching in neonatal B cell,the number of immunoglobulin secreting cells (IgSCs) in human cord blood mononuclear cell and in cord blood B cell after addition Of recombinant human cytokines was investigated by using reverse enzyme-linked immunospot assay.Results showed that normal cord mononuclear cells could produce only a few number of IgSCs.However,cord blood B cells were induced to secret IgA,IgG and IgM after activated by the anti-CD3-monoclonal antibody,neonatal cord blood T cells treated by mitomycin C,and also by rIL-2,rIL-4,rIL-10 or their combinations. These results suggested that supplemental cytokines is likely to contribute to the immunoglobulin class switching in neonatal B cell in vitro.

关 键 词：脐血 B细胞 免疫球蛋白 白细胞介素 

分 类 号：R446.62[医药卫生—诊断学]