羰基还原酶与甲酸脱氢酶偶联催化制备(R)-苯基乙二醇  

Preparation of(R)-1-Phenyl-1,2-ethanediol by Biotransformation with a Coupled System of(R)-Specific Carbonyl Reductase and Formate Dehydrogenase

作　　者：张荣珍[1] 徐岩 耿亚维[1] 王珊珊[1] 孙莹[1] 

机构地区：[1]江南大学生物工程学院教育部工业生物技术重点实验室,江苏无锡214122

出　　处：《过程工程学报》2009年第4期801-805,共5页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:20776060);国家重点基础研究发展规划(973)基金资助项目(编号:2003CB716008);国家高技术研究发展计划(863)基金资助项目(编号:2007AA02Z200,2006AA020104);新世纪优秀人才支持计划资助项目(编号:NCET-04-0498)

摘　　要：以重组菌E.coli Rosetta/pETDuet-rcr-fdh全细胞为催化剂,2-羟基苯乙酮和甲酸钠为双底物,研究了生物催化反应体系中温度、pH值、底物2-羟基苯乙酮的初始浓度、反应时间、细胞浓度和状态等对产物(R)-苯基乙二醇生成效率的影响规律.结果表明,在温度35℃和pH7.0、底物初始浓度6g/L、时间36h、湿细胞浓度10%(ω)的条件下,产物的光学纯度高达98.37%e.e.,得率达79.14%.在上述反应体系中添加5mmol/LZnSO4后,重组菌催化的不对称还原反应效率显著提高,产物(R)-苯基乙二醇光学纯度达到100%e.e.,得率高达86.3%.The effects of reaction temperature,pH value,initial substrate concentration,reaction duration,cell concentration and forms on biotransformation reaction for preparation of（R）-1-phenyl-1,2-ethanediol were studied with 2-hydroxyacetophenone and sodium formate as substrates and the whole cells of E.coli Rosetta/pETDuet-rcr-fdh as catalyst.The results showed that when the concentration of 2-hydroxyacetophenone was 6 g/L,（R）-1-phenyl-1,2-ethanediol was produced with high optical purity of 98.37% enantiomeric excess and yield of 79.14% at the conditions of temperature 35 ℃,pH 7.0,reaction duration 36 h and wet cell concentration 10%（ω）.Further research showed that the biotransformation efficiency could be well improved by addition of Zn2＋.In the presence of Zn2＋ with a final concentration of 5 mmol/L,the enantiomeric excess and yield reached 100% and 86.3%,respectively.

关 键 词：重组大肠杆菌 生物催化 (R)-苯基乙二醇 光学纯度 得率 

分 类 号：Q814.4[生物学—生物工程]