A novel biosynthetic hybrid scaffold seeded with olfactory ensheathing cells for treatment of spinal cord injuries  

作　　者：QIAN Lei-min ZHANG Zhi-jian GONG Ai-hua QIN Ru-juan SUN Xiang-lan CAO Xu-dong LIU Jin-bo JIANG Ping CHEN Yong-chang 

机构地区：[1]Department of Gastroenterology, People's Hospital of Jiangyin,Wuxi, Jiangsu 214400, China [2]Department of Histology and Embryology, School of Medicine,Jiangsu University, Zhenjiang, Jiangsu 212001, China [3]Institute of Molecular and Cellular Biology, Academy of Science,Jiangsu University, Zhenjiang, Jiangsu 212001, China [4]Department of Chemical Engineering, University of Ottawa, 161Louis Pasteur, Ottawa, Ontario, Canada

出　　处：《Chinese Medical Journal》2009年第17期2032-2040,共9页中华医学杂志（英文版）

基　　金：This study was supported by grants from the National Natural Science Foundation of China （No. 30570981 and No. 30571878）.

摘　　要：Background Implantation of tissue-engineered scaffolds is one of the most promising therapeutic strategies for inducing nerve regenerations following spinal cord injuries. In this paper, we report a novel bioengineered hybrid scaffold comprised of three major extracellular matrix （ECM） proteins. Methods ECM-scaffolds （ECM-S） were prepared by gelling fibrinogen, fibronectin and laminin using fresh rat plasma. Olfactory ensheathing cells （OECs） were isolated from fresh rat olfactory mucosa, purified under differential adhesion, and assessed by immunofluorescent staining. OECs were seeded onto ECM-S and cultured. The effects of the scaffolds on the seeded cells were detected using the immunofluorescent staining, Western blotting, scanning electron microscopy and transmission electron microscopy. Results Tissue-engineered ECM-S could be easily molded into mat-like or cylindrical shapes and gelled by addition of fresh plasma. Observations by electron microscopy show that the ECM-S forms a stable three-dimensional porous network. Studies on the effects of the ECM-S on the biological behaviors of OECs in vitro indicate that the scaffold can promote OEC adhesion, proliferation and process extensions. Additionally, OECs seeded on the scaffold maintained the expression of nerve growth factor, matrix metalloproteinase-3 and matrix metalloproteinase-9. Conclusion We developed a biosynthetic hybrid gel which could be used as a scaffold for OEC transplantation; this gel can promote nerve regeneration following spinal cord injuries. Chin Med J 2009; 122（17）：2032-2040Background Implantation of tissue-engineered scaffolds is one of the most promising therapeutic strategies for inducing nerve regenerations following spinal cord injuries. In this paper, we report a novel bioengineered hybrid scaffold comprised of three major extracellular matrix （ECM） proteins. Methods ECM-scaffolds （ECM-S） were prepared by gelling fibrinogen, fibronectin and laminin using fresh rat plasma. Olfactory ensheathing cells （OECs） were isolated from fresh rat olfactory mucosa, purified under differential adhesion, and assessed by immunofluorescent staining. OECs were seeded onto ECM-S and cultured. The effects of the scaffolds on the seeded cells were detected using the immunofluorescent staining, Western blotting, scanning electron microscopy and transmission electron microscopy. Results Tissue-engineered ECM-S could be easily molded into mat-like or cylindrical shapes and gelled by addition of fresh plasma. Observations by electron microscopy show that the ECM-S forms a stable three-dimensional porous network. Studies on the effects of the ECM-S on the biological behaviors of OECs in vitro indicate that the scaffold can promote OEC adhesion, proliferation and process extensions. Additionally, OECs seeded on the scaffold maintained the expression of nerve growth factor, matrix metalloproteinase-3 and matrix metalloproteinase-9. Conclusion We developed a biosynthetic hybrid gel which could be used as a scaffold for OEC transplantation; this gel can promote nerve regeneration following spinal cord injuries. Chin Med J 2009; 122（17）：2032-2040

关 键 词：tissue-engineering scaffold biological behaviors extracellular matrix olfactory ensheathing cells 

分 类 号：Q255[生物学—细胞生物学] Q426