精液质量对猪胚胎体外生产的影响研究  

Effect of semen quality on in vitro production of porcine embryos

作　　者：葛立军[1] 卢庆[1] 高俊锋[1] 曹爱巧[1] 郑筱峰[1] 芮荣[1] 

机构地区：[1]南京农业大学动物医学院,南京210095

出　　处：《上海农业学报》2009年第3期6-9,共4页Acta Agriculturae Shanghai

基　　金：农业部"十一五"科技支撑计划(2008BADB2B11);国家高技术研究与发展计划(2008AA101003)资助

摘　　要：以猪鲜精、冻精进行体外受精试验。结果表明:用劣质鲜精(活力≤0.5、畸形率>30%)进行体外受精后,所获卵裂率与优质鲜精组(活力>0.8、畸形率<15%)差异不显著(51.0%对53.6%),但16-细胞胚发育率较优质鲜精组明显降低(28.9%对47.4%)。用不同精卵比(A组12 000∶1、B组6 000∶1和C组3 000∶1)的冻精进行体外受精发现,A组卵裂率(37.5%)和16-细胞胚发育率(34.4%)均明显低于B、C组,后2组卵裂率和16-细胞胚发育率无明显差异(54.3%对54.2%,48.5%对45.7%)。以活力较高(>0.5)的冻精进行体外受精后,所获卵裂率和16-细胞胚发育率与鲜精组无明显差异(54.3%对51.1%,48.2%对45.4%);而Ⅱ组(冻精活力≤0.4)的体外受精卵裂率虽与Ⅰ组(冻精活力>0.5)和对照组无显著差异,但16-细胞胚发育率仅为37.0%,明显低于Ⅰ组的48.2%和对照组的45.4%。试验结果表明:精液质量会影响体外受精胚胎的后续发育能力。The in vitro fertilization （IVF） experiment was conducted with fresh and frozen boar semens to study the effect of semen quality on in vitro production of porcine embryos. The results showed that after IVF, the fresh semen of inferior quality （vigor≤0.5, morphological abnormality〉 30%） was not significantly different from the fresh semen of superior quality （vigor〉0.8,morphological abnormality〈15%） in cleavage rate （51.0% to 53.6% ） but significantly lower than the fresh semen of superior quality in 16-cell embryonic development rate （28.9% to 47.4%）. The IVF of frozen semens at different sperm-oocyte ratios （Group A： 12 000 ： 1, Group B. 6 000 ： 1 and Group C. 3 000 ： 1） revealed that Group A had significantly lower cleavage rate （37.5%） and 6-cell embryonic development rate （34.4%） than Group B and Group C,but between Group B and Group C there were not significant differences in both rates （54.3% to 54.2% ,48.5% to 45.7%）. The IVF of frozen semen with rather high vigor （〉0.5） was not significantly different from that of fresh semen in both rates （54.3% to 51.1%,48.2% to 45.4%）. While the IVF of Group Ⅱ （vigor of frozen semen,0.4） was not significantly different from that of Group Ⅰ （vigor〉0.5） and control group in cleavage rate,the 16-cell embryonic development rate of Group Ⅱ was only 37.0% and significantly lower than those of Group Ⅰ （48.2%） and control group （45.4%）. This study confirmed that the quality of boar semen would impact the subsequent developmental capacity of IVF embryos.

关 键 词：猪 精液 冷冻 体外受精 胚胎发育 

分 类 号：S828[农业科学—畜牧学] S814.8[农业科学—畜牧兽医]