芹菜素分子印迹聚合物的合成及谱学研究  

Preparation and spectral characterization of apigenin molecularly imprinted polymer

作　　者：李丽丽[1] 陈小明[1] 

机构地区：[1]湘潭大学化学学院,湖南湘潭411105

出　　处：《药学学报》2009年第8期868-872,共5页Acta Pharmaceutica Sinica

摘　　要：采用分子印迹技术合成芹菜素分子印迹聚合物。紫外光谱法证明了模板分子与功能单体之间存在相互作用,据此选择了聚合反应合适的溶剂并推测出聚合物的印迹机制;傅立叶红外光谱仪测定了聚合物的结构特征;平衡结合实验研究了聚合物的吸附特性。结果表明,在所研究的浓度范围内(0.1～2.5mmol·L-1),印迹聚合物的吸附量大于空白聚合物的吸附量,由Scatchard分析得聚合物对芹菜素的识别主要有两类结合位点,其离解常数KD和最大表观结合量Qmax分别为2.52×10-4、0.54×10-3mmol·L-1和2.65、18.89μmol·g-1。分子印迹聚合物呈现出空白聚合物没有的高选择性。An apigenin molecularly imprinted polymer was prepared by bulk polymerization using apigenin as template, acrylamide （AA） as a functional monomer, ethylene glycol dimethacrylate （EDMA） as a crosslinking agent, acetonitrile and N, N-dimethylformamide as porogenic solvents, and 2, 2＇-azobisisobutyronitrile （AIBN） as an initiator. The interaction between template and functional monomer was proved by ultraviolet visible （UV） spectrophotometry and based on the results, appropriate reaction solvent was selected and the synthesizing process was estimated. The molecularly imprinted polymer structure was analyzed by Fourier Transform Infrared （FT-IR） spectrophotometry. The molecularly imprinted polymer was investigated in equilibrium binding experiment to evaluate its adsorption property, the results showed that the adsorption of apigenin on molecularly imprinted polymer is higher than that on blank polymer in the studied concentration range （0.1 - 2.5 mmol·L^-1）. Scathcard analysis showed that two classes of binding sites existed in the apigenin imprinted polymers, with their KD and Qmax estimated to be 2.52×10^-4, 0.54×10^-3 mmol·L^-1 and 2.65, 18.89 μmol·g^-1, respectively. Molecularly imprinted polymer showed higher affinity than blank polymer.

关 键 词：分子印迹聚合物 芹菜素 紫外光谱 红外光谱 Scatchard分析 

分 类 号：O657.3[理学—分析化学]