SLC及其受体CCR7在胰腺癌中的表达及与淋巴管生成相关性的研究  

Secondary lymphoid-tissue chemokine and CCR7 in it＇s relation with lymphangiogenesis of pancreatic adenocarcinoma

作　　者：赵文华[1] 王长亮[1] 崔凯[1] 张波[1] 周武元[1] 张红雨[2] 仲伟霞[1] 李胜[1] 

机构地区：[1]山东省肿瘤防治研究院肝胆外科,济南250117 [2]山东大学齐鲁医院老年病科

出　　处：《中华普通外科杂志》2009年第8期658-662,共5页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目（30571712）;山东省自然科学基金资助项目（Y2007F06）;山东省科技发展计划资助项目（20070GG20002022）

摘　　要：目的研究胰腺癌中次级淋巴组织趋化因子（SLC）及其受体CCR7的表达情况，探讨其在胰腺癌淋巴管生成中的作用。方法采用免疫组织化学和RT—PCR方法检测30例胰腺癌病灶、癌旁组织、正常胰腺和胰周淋巴结中的中SLC、CCR7的表达情况，形态计量学图像分析法测定胰腺癌中微淋巴管密度（MLVD），分析其表达与胰腺癌MLVD间的关系。结果SI。C蛋白在胰腺癌组织、癌旁组织、胰周淋巴结、正常胰腺组织中的阳性率分别为16．7％、43．3％、46．6％和76．7％。CCR7蛋白在上述组织中阳性率分别为76．7％、66．7％、70．0％和30．O％；RT-PCR表明SLC、CCR7 mRNA表达与其蛋白表达强度相同。胰腺癌SLC阳性组与阴性组中MLVD无明显差异（P〉0．05），CCR7阳性组中MLVD明显高于阴性组（P=0．004）。结论SLC与胰腺癌的分期及转移无关，CCR7的表达与胰腺癌TNM分期和淋巴结转移密切相关，其高表达对胰腺癌中淋巴管生成及淋巴结转移可能起促进作用。Objective To study the expression of secondary lymphoid-tissue chemokine （SLC）, CCR7 and its correlation with clinical pathology and lymphangiogenesis in pancreatic adenocarcinoma （PAC）. Methods The tissue specimens including PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lymph nodes were obtained from 30 patients with PAC. The expressions of SLC and CCR7 in these tissues were assayed by immunohistochemical staining and reverse transcription polymerase chain reaction （RT-PCR）. MLVD marked by VEGFR-3 was detected by morphometric analysis, and the relationship between MLVD and clinical pathology of PAC was analyzed. Results In all the specimens, the positive rates of SLC protein in PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lymph nodes were respectively 16. 7% , 43.3% , 76.7% and 46. 6%. The positive rates of CCR7 protein in PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lymph nodes were respectively 76. 7%, 66. 7%, 30. 0% and 70. 0%. The results of RT-PCR and fluorescence quantitative real-time PCR indicated that the expression levels of CCR7 mRNA in PAC tissues, the cancerous peripheral tissues and peripheral lymph nodes were higher than that in the normal pancreatic tissues （ P 〈 0.01 ）. There was no significant correlation hetween the expression of SLC protein with MLVD of PAC （P 〉 0. 05）. There was 23 specimens that the CCR7 protein was positive, and among these specimens the MLVD was higher than that in negative group of CCR7 protein （P = 0.004 ）. Conclusions The expression of SLC was not related to lymphatic metastasis and TNM stages of PAC. The expression of CCR7 was significantly associated with lymphatic metastasis and TNM stages of PAC, and the high expression of CCR7 in PAC tissues was significantly associated with lymphangiogenesis of PAC.

关 键 词：胰腺肿瘤 趋化因子类 淋巴管生成 

分 类 号：R686[医药卫生—骨科学]