鲍氏不动杆菌氨基糖苷类修饰酶基因类型分析  

Genotypes of Aminoglycoside-modifying Enzymes of Acinetobacter baumannii

作　　者：刘平[1] 叶惠芬 陈惠玲 丁群力[1] 

机构地区：[1]广州市第一人民医院中心ICU [2]检验科,广东广州510180

出　　处：《中华医院感染学杂志》2009年第17期2226-2229,共4页Chinese Journal of Nosocomiology

摘　　要：目的鲍氏不动杆菌(ABA)氨基糖苷类药物修饰酶(AMEs)基因类型。方法采用K-B药敏法测定2003-2006年临床分离的ABA对庆大霉素、阿米卡星、妥布霉素的药敏表型,筛选出23株对氨基糖苷类药物耐药的菌株,以聚合酶链反应(PCR)的方法对9种AMEs进行检测和分析。结果ABA对庆大霉素的耐药率为86.96%,对妥布霉素的耐药率为69.56%,对阿米卡星的耐药率为56.5%;9种AMEs检出率由高到低分别为:ant(3′)-Ⅰ(69.56%)、aac(3)-Ⅰ(60.87%)、aac(6′)-Ⅰ(56.52%)、aph(3′)-Ⅵ(47.82%)、aac(3)-Ⅱ(30.4%)、aac(6′)-Ⅱ(26.09%)、ant(2″)-Ⅰ(21.73%)。结论ABA对氨基糖苷类抗菌药的耐药主要由AMEs引起。OBJECTIVE To investigate the genotypes of aminoglycoside-modifying enzymes （AMEs） genes of Acinetobacter baumannii. METHODS Clinical isolates of A. baumannii were collected from 2003 to 2006, and their resistance to gentamicin,amikacin and tobramycin were tested by K-B method. Twenty-three isolates were chosen because of their resistance to aminoglycoside antibiotics （at least resistant to one kind of the drugs）. Nine types of the AMEs were detected by PCR. RESULTS Drug resistant rates of 23 isolates of A. baumannii to gentamicin, amikacin and tohramycin, were 86. 96%, 56.5% and 69.56% ,respectively. The detection rates of the 9 AMEs, including ant（3′）-Ⅰ ,aac（3）-Ⅰ .aac（6′）-Ⅰ , aph（3′）-Ⅵ, aac（3）-Ⅱ , aac（6′）-Ⅱ and ant（2″）-Ⅰ were 69.56%, 60.87%, 56. 52%, 47. 82%, 30. 4%, 26.09% and 21. 73%, respectively. CONCLUSIONS The resistance to aminoglycoside antibiotics of A. baumannii is mainly caused by AMEs.

关 键 词：鲍氏不动杆菌 氨基糖苷类修饰酶 基因类型 

分 类 号：R378[医药卫生—病原生物学]