嗜麦芽寡养单胞菌氨基糖苷类修饰酶与Ⅰ类整合酶基因的研究  

Aminoglycosides Modifying Enzyme Genes and intⅠ gene in Stenotrophomonas maltoplilia

作　　者：邓笑伟[1] 张丹梅[1] 刘长庭[2] 周红莉[1] 李爱玲[1] 

机构地区：[1]武警总医院干二科,北京100039 [2]解放军总医院南楼呼吸科,北京100853

出　　处：《中华医院感染学杂志》2009年第17期2233-2235,共3页Chinese Journal of Nosocomiology

基　　金：武警总医院科研课题(W22008010);北京市科委重大培育专项课题(Z0005190041891)

摘　　要：目的了解医院嗜麦芽寡养单胞菌氨基糖苷类修饰酶和Ⅰ类整合酶基因存在的情况。方法收集2006年1月-2007年10月武警总医院住院患者标本中分离到的嗜麦芽寡养单胞菌27株,微量肉汤释释法测定14种抗菌药物的敏感性;PCR方法对嗜麦芽寡养单胞菌进行氨基糖苷类修饰酶和Ⅰ类整合酶基因检测。结果嗜麦芽寡养单胞菌对14种抗菌药物耐药严重,27株嗜麦芽寡养单胞菌氨基糖苷类修饰酶基因ant(2″)-Ⅰ阳性5株(18.5%),aac(3)-Ⅱ3株(11.1%)、aac(6′)-Ⅱ1株(3.7%)、Ⅰ类整合酶(intⅠ)阳性8株(29.6%)。结论嗜麦芽寡养单胞菌对氨基糖苷类耐药的主要原因是ant(2″)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅱ,携带Ⅰ类整合子可能是导致其对氨基糖苷类耐药性高的原因。OBJECTIVE To study the aminoglycosides modifying enzyme genes and int Ⅰ gene in Stenotrophomonas maltophilia in Chinese Armed Police Forces General Hospital. METHODS The samples of 27 multi-resistant S. maltophilia were collected from inpatiens from Jan 2006 to Oct 2007 in this Hospital. The sensitivity of the isolates to 14 antibacterial agents was determined using a broth induction method. The aminoglycosides modifying enzyme genes and int Ⅰ 1 gene were detected by PCR. RESULTS The multi-drug resistance of S. magtophilia was a serious problem. In 27 strains of S. maltophilia, the positive ant（2″）- Ⅰ were in 5 strains （18.5M）, aac（3）-Ⅱ in 3 strains （11. 1%） and aac（6′）-Ⅱ in 1 strain （3. 7%）. The positive int Ⅰ gene was found in 11 strains （29.6 %）. CONCLUSIONS Multi-resistant S. maltophilia resistant to aminoglycosides mainly due to the presence of aminoglyeoside modifying enzymes ant （2″）- Ⅰ , aac （3）- Ⅱ and aac （6′）- Ⅱ. The aminoglycoside modifying enzymes ant（3′）-Ⅰ and aac（6）-Ⅰ Z were not detected carrying Int I would be the reason of S. maltophilia resistant to aminoglycosides.

关 键 词：嗜麦芽寡养单胞菌 氨基糖苷类修饰酶 Ⅰ类整合酶基因 多药耐药 

分 类 号：R378[医药卫生—病原生物学]