HBV感染血清DNA、HBeAg、PreS1检测结果分析及意义  

Detecting Results of HBV-DNA,HBeAg and PreS1 of HBV Infected Sera:Analysis and Significance

作　　者：陈黎亚 赵升 王茂峰 万汝根 

机构地区：[1]东阳市人民医院检验科,浙江东阳322100

出　　处：《中华医院感染学杂志》2009年第17期2250-2251,共2页Chinese Journal of Nosocomiology

摘　　要：目的探讨分析HBV感染者血清HBV-DNA、HBeAg、PreS1检测结果及临床意义。方法用酶联免疫法(ELISA)对450例HBV感染者血清进行乙型肝炎5项标志物和PreS1检测,用荧光定量PCR法检测HBV-DNA。结果450例HBV感染者血清HBV-DNA、HBeAg及PreS1的阳性率分别为74.4%、48.9%、63.3%;285例PreS1阳性标本中HBeAg、HBV-DNA的阳性数分别为190、270例,PreS1与HBeAg、HBV-DNA二者间差异有统计学意义(P<0.01),PreS1与HBV-DNA符合率较HBeAg高;220例HBeAg阳性标本中PreS1、HBV-DNA的阳性数分别为190、200例,PreS1、HBV-DNA与HBeAg呈正相关,三者差异无统计学意义(P>0.05)。结论PreS1与HBV-DNA比HBeAg更敏感,PreS1与HBV-DNA相关性较好,反映了HBV病毒感染及复制情况;三者联合检测在乙型肝炎诊断和治疗中具有重要的临床应用价值。OBJECTIVE To detect and analyze the results and significance of HBV-DNA, HBeAg and PreS1 of HBV infective sera. METHODS Routine detection and PreS1 antigen of 450 sera were tested by ELISA, and HBVDNA was detected by fluorescent quantitation PCR. RESULTS The positive rates of HBV-DNA, HBeAg and PreS1 were 74.4%, 48.9% and 63.3%, respectively, in 450 cases of HBV infective sera. Among 285 PreS1- positive samples, the positive cases of HBeAg and HBV-DNA were 190 and 270, respectively. There were significant difference （P（0.01） between PreS1 and HBeAg, HBV-DNA. The concordance rates of PreS1 and HBV-DNA were higher than HBeAg. The positive rates of PreS1 and HBV-DNA in 220 cases of HBeAg-positive samples were 190 and 200, respectively. Meanwhile, there weren＇t significant difference （P^0. 05） among PreS1, HBV-DNA and PreS1, and they three were in positive correlation. CONCLUSIONS PreS1 and HBV-DNA are more sensitive than HBeAg, and PreS1 is better coincided with HBV-DNA. They can reflect the infection and replication condition of HBV. Therefore, it has important clinical meaning for the diagnosis and therapy of HBV to simultaneously used HBV-DNA, HBeAg and PreS1.

关 键 词：HBV-DNA 荧光定量PCR 乙型肝炎E抗原 前S1蛋白 乙型肝炎病毒 

分 类 号：R512.62[医药卫生—内科学]