重症监护病房鲍氏不动杆菌感染暴发的监控  

Surveillance of Acinetobacter baumannii Outbreak in ICU

作　　者：张亚莉[1] 耿穗娜[2] 孙树梅[1] 姚翠军[1] 汪能平[1] 

机构地区：[1]南方医科大学南方医院感染管理科,广东广州510515 [2]南方医科大学南方医院检验科,广东广州510515

出　　处：《中华医院感染学杂志》2009年第17期2252-2254,共3页Chinese Journal of Nosocomiology

摘　　要：目的了解某重症监护病房(ICU)一周内发生的医院感染,与病房环境中的多药耐药鲍氏不动杆菌的耐药谱特征和同源性。方法用K-B法检测17株鲍氏不动杆菌的耐药情况;用随机引物扩增多态DNA(RAPD)检测并进行同源性分析。结果病房内环境分离出12株鲍氏不动杆菌,其中人携带分离出3株;ICU内物体表面分离出9株,其中11株为多药耐药菌,且耐药表型与患者的5株感染菌完全一致;RAPD分析患者感染菌3株、环境分离菌8株为同一基因型。结论该病房存在着某一基因型鲍氏不动杆菌感染暴发流行。OBJECTIVE To survey the hospital infection of Acinetobacter baurnannii in an ICU during one week＇s time, and study its antibiotic resistance pattern and homogeneity. METHODS The antibiotic resistance patterns of 17 A. baumannii strains were obtained by K-B method, and their homogeneity information was determined using random amplified polymorphic DNA （RAPD）. RESULTS Twelve strains of A. baurnannii were isolated from the ICU, among which 3 were isolated from patients, and 9 were isolated from the surface of facilities in the ICU. Eleven strains were found to be multi-antibiotic resistant, and their resistance patterns were the same with the 5 strains from patients. RAPD results showed that the 3 strains from patients and 8 strains from the environment had the same genotype. CONCLUSIONS A small outbreak of a genotype of A. baurnannii infection happens in the ICU.

关 键 词：鲍氏不动杆菌 感染暴发 随机引物扩增多态性DNA 

分 类 号：R378[医药卫生—病原生物学]