缬沙坦对氧化低密度脂蛋白诱导的人内皮细胞基质金属蛋白酶-1表达的影响  

Effect of valsartan on expression of matrix metalloproteinase-1 in cultured human umbilical vein endothelial cells induced by ox-LDL

作　　者：姜立清[1] 汤楚中[2] 胡大一[2] 

机构地区：[1]北京市昌平区医院心内科,北京102200 [2]北京大学人民医院心脏中心

出　　处：《中华老年心脑血管病杂志》2009年第9期731-732,共2页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的观察缬沙坦对氧化低密度脂蛋白(ox-LDL)诱导的人内皮细胞基质金属蛋白酶-1(MMP-1)表达的影响。方法体外培养人脐静脉内皮细胞,待细胞生长到融合状态时,将细胞分为4组:对照组;OX-LDL组(加ox-LDL 100 mg/L);低浓度组(先加ox-LDL 100 mg/L,再加缬沙坦10 μmol/L);高浓度组(先加ox-LDL 100mg/L,再加缬沙坦30μmol/L)。RT-PCR测定MMP-1 mRNA的表达。结果与对照组比较,ox-LDL组MMP-1mRNA的表迭明显增多(P<0.05),与ox-LDL组比较,低、高浓度组抑制MMP-1 mRNA的表达(P<0.05),并呈浓度依赖性。结论缬沙坦的抗炎及稳定动脉粥样硬化斑块的作用,可能与抑制内皮细胞MMP-1的表达有关。Objective To investigate the effects of valsartan treatment on expression of matrix metalloproteinase-l（MMP-1） in cultured HUVECs induced by ox-LDL. Methods HUVECs grew to confluence and were divided into 4 groups, control group, ox-LDL （100 mg/L） group, ox-LDL -k valsartan 10 μmol/L group and ox-LDL ＋valsartan 30 μmol/L group. Expression of MMP-1 mRNA was determined by RT-PCR. Results Compared with control group,expression of MMP-1 mRNA was increased in ox-LDL group （P 〈 0.05）. Valsartan dose-dependently decreased the expression of MMP-1 mRNA in ox-LDL-induced HUVECs. Conclusions Valsartan has anti-inflammatory and plaque stabilization ability which may be related to the inhibition of expression of MMP-1 mRNA in endothelial cells.

关 键 词：脂蛋白类 LDL 内皮细胞 间质胶原酶 受体 血管紧张素 

分 类 号：R543.5[医药卫生—心血管疾病] R979.1[医药卫生—内科学]