文蛤多肽对体外培养宫颈癌Hela细胞的抑制作用  

Effect of the Polypeptides from Meretrix meretrix Linnaeus on Proliferation of Cervical Cancer Hela Cells

作　　者：张剑[1] 康劲翮[1] 刘凤娇[1] 范成成[1] 李华亮[1] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2009年第5期729-732,共4页Journal of Xiamen University：Natural Science

基　　金：厦门市科技计划项目(3502Z20063021);厦门大学科技创新工程基金(XDKJCX20043001)资助

摘　　要：采用蒸馏水抽提,有机溶剂沉淀大分子蛋白,SephadexG-25分子筛柱层析等技术从文蛤肉分离纯化,得到一种低分子量的蛋白多肽,命名为Mer2.实验结果表明,Mer2对宫颈癌Hela细胞有明显的抑制效应,Mer2对宫颈癌Hela细胞生长的抑制作用呈量效与时效关系.经40μg/mLMer2处理,体外培养宫颈癌Hela细胞生长缓慢,细胞形态发生明显改变,细胞生长抑制率达78.1%,并出现明显的凋亡峰,凋亡率为25.5%.An anticancer peptide was purified from Mercenaria（Meretrix meretrix Linnaeus）by method of fragmentation with distilled water,organic precipitation and column chromatogram （Sephadex G-25）. The anticancer peptide was obtained and named as Met2. The antitumor activity of Mer2 was preliminarily determined with the MTT method in vitro. Mer2 had strong inhibitory effect on growth of Human cervial cancer cell strains Hela,in a dose-dependent manner. It was found that the Hela cells,after it was treated by 40 μg/mL of Mer2, grew slower than that of the control, and the shapes had been changed distinctly. The inhibitory rate of the cell growth was determined to be 78.1%. In the mean time,low cytometry analysis showed that the apoptosis rate of the cells was up to 25.5%.

关 键 词：文蛤多肽 宫颈癌HELA细胞 抗癌 MTT比色法 凋亡 

分 类 号：Q356.1[生物学—遗传学]