梨属植物基因组DNA提取部位对RAPD扩增的影响  

Effect of Different Tissues in Pyrus L. for Genome DNA Extract on RAPD

作　　者：马艳芝[1,2] 张玉星[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]唐山师范学院生命科学系,河北唐山063000

出　　处：《西南农业学报》2009年第4期1042-1045,共4页Southwest China Journal of Agricultural Sciences

基　　金：河北省自然科学基金"梨属种质资源分子遗传连锁图谱构建与遗传多样性分析"(303240)

摘　　要：中国梨属植物资源丰富,RAPD技术已广泛用于梨资源遗传多样性研究。通过对不同部位提取的DNA纯度进行测定,并进行RAPD扩增分析,为RAPD技术在梨属种质资源中的应用提供参考。以鸭梨和杜梨为试验材料,利用CTAB法对其幼叶、老叶及韧皮部等不同组织或同一组织的不同时期进行了基因组DNA的提取,并对其进行了RAPD反应的比较。所提取的鸭梨的幼嫩叶片、成熟叶片、韧皮部DNAOD260/OD280的比值分别为2.0、1.8、1.7;杜梨幼嫩叶片、成熟叶片、韧皮部DNAOD260/OD280的比值分别为1.9、1.8、1.6。二者都能成功进行RAPD扩增,且扩增结果基本一致。利用CTAB法从鸭梨和杜梨的幼嫩叶片、成熟叶片、韧皮部提取的DNA均能成功进行RAPD扩增,且结果基本一致。RAPD technique was applied on study of germplasm genetic diversity. In order to provide RAPD information to application of Pyrus germplasmic rosources, Yali pear and Betulaefolia were tested. Genome DNA of the pears was extracted from different tissues or different phases of the same tissue, such as new leaves, old leaves and phloem by CTAB method, and checked by RAPD amplification. The results showed that OD260/OD280 of the DNA both from leaves and phloem were among 1.6 - 2.0, and all of them could be amplified successfully by RAPD and with the same amplification effect.

关 键 词：梨属 不同部位 基因组DNA RAPD 

分 类 号：S188[农业科学—农业基础科学]