新近发生扩张型心肌病患者外周血淋巴细胞CD4^＋/D8^＋比值的测定及其与左室射血分数的相关性  

The correlation of peripheral blood T-lymphocyte CD4^＋/D8^＋ with left ventricular ejection fraction in patients with recent-onset dilated cardiomyopathy

作　　者：陆玉良[1] 杨向军[2] 

机构地区：[1]湖州市中心医院心内科,浙江湖州313000 [2]苏州大学附属第一医院心内科

出　　处：《浙江医学》2009年第8期1084-1085,1089,共3页Zhejiang Medical Journal

摘　　要：目的探讨新近发生的扩张型心肌病患者外周血淋巴细胞CD4^＋/D8^＋比值同左室射血分数的相关性。方法对25例新近发生的扩张型心肌病患者（心肌病组）和22例体检健康者（对照组）通过流式细胞仪测定外周血淋巴细胞CD4^＋和CD8^＋的值，测算出CD4^＋/D8^＋比值，同时采用心脏超声测定扩张型心肌病患者的左室射血分数，对两组对象的上述参数作对比分析，并对心肌病组患者CD4^＋/D8^＋比值与左室射血分数行直线相关分析。结果心肌病组患者淋巴细胞CD8^＋值明显高于对照组（22.35±2.43 vs 20.49±2.18，P〈0.05），CD4^＋/D8^＋比值显著低于对照组（1.90±0.27 vs 2.16±0.28，P〈0.01），但两组间CD4^＋值并无明显差异（41.96±3.57 vs 43.81±3.59，P〉0.05）。心肌病组患者CD4^＋/D8^＋比值与左室射血分数呈显著负相关（r=-0.65,P〈0.01）。结论新近发生的扩张型心肌病患者，其CD4^＋ T淋巴细胞水平与健康成人相似，但CD8^＋ T淋巴细胞水平则明显下降。其心功能下降与免疫功能紊乱相关。Objective To investigate the correlation of the T-cell CD4^＋/D8^＋ ratio with left ventricular ejection fraction in patients with recent-onset dilated cardiomyopathy （DCM）. Methods Twenty-five patients with recent-onset dilated cardiomyopathy （≤6 months of symptoms） and 22 healthy individuals were enrolled in the study. Peripheral blood T-lymphocyte CD4^＋/D8^＋ ratio was measured by flow cytometry, and echocardiography was performed for assessment of left ventricular ejection fraction （LVEF）. The relationship between CD4^＋/D8^＋ ratio and the left ventricular ejection fraction was analyzed by simple linear correlation. Results There was no significant difference in CD4^＋ count between DCM and control groups （t=1.7675, P〉0.05）; while the CD8^＋ in DCM group were lower than those in control group （t=2.7662, P〈0.05）. The CD4^＋/D8^＋ ratio in DCM group was higher than that in control group （t=3.2298, P〈0.01）. The CD4^＋/D8^＋ ratio was negatively correlated with left ventricular ejection fraction in DCM group （Y=0.6014-0.1366X, r=-0.65, t=4.1355, P〈0.01）. Conclusion Peripheral blood T-cell CD4^＋/D8^＋ is decreased in patients with dilated cardiomyopathy, and CD4^＋/D8^＋ ratio is correlated with left ventricular ejection fraction.

关 键 词：扩张型心肌病 免疫功能 T淋巴细胞亚群 心功能 流式细胞术 

分 类 号：R542.2[医药卫生—心血管疾病] R593.241[医药卫生—内科学]