罗格列酮对OX-LDL诱导的大鼠肾小球系膜细胞增殖及分泌细胞外基质的影响  

Effect of rosiglitazone on proliferation of rat mesangial cells and synthesis of extracellular matrix induced by ox-LDL

作　　者：杜月光[1] 柴可夫[1] 赵天喜[1] 

机构地区：[1]浙江中医药大学基础医学院病理生理教研室,浙江杭州310053

出　　处：《浙江医学》2009年第9期1205-1207,共3页Zhejiang Medical Journal

基　　金：浙江省自然科学基金资助项目（M303727）.

摘　　要：目的观察罗格列酮对氧化低密度脂蛋白（ox-LDL）诱导的肾小球系膜细胞（RMC）增殖和细胞外基质（ECM）分泌的影响。方法采用RT-PCR法检测正常对照组、ox-LDL组、罗格列酮组、罗格列酮＋ox-LDL组RMC内的转化生长因子β1（TGFβ1）mRNA表达；同时采用MTT法检测各组RMC增殖水平，并予ELISA技术检测上清液中TGFβ1、层粘连蛋白（FN）、Ⅳ型胶原（ColⅣ）蛋白的含量。结果罗格列酮＋ox-LDL组RMC增殖水平较ox-LDL组降低，且RMC表达的TGFβ1 mRNA水平和分泌的TGFβ1、FN、ColⅣ蛋白水平均较ox-LDL组降低（均P〈0.05或0.01）。结论罗格列酮可通过抑制ox-LDL诱导的RMC增殖和ECM及TGFβ1的分泌，从而发挥抗肾纤维化作用。Objective To investigate the effect of rosiglitazone on the proliferation of rat renal mesangial cells （RMCs） and synthesis of extracellular matrix induced by ox-LDL. Methods TGF- β1 mRNA expression of RMC in control group, ox-LDL group, rosiglitazone group and rosiglitazone＋ox-LDL group were measured by RT-PCR. The cell proliferation was measured by MTT method. Synthesis of TGFβ1,FN,Col Ⅳ in cultured mesangial cells were determined by ELISA methods. Results In rosiglitazone＋ox-LDL group, RMC proliferation was lower, compared with ox-LDL group. The TGFβ1 mRNA and protein levels of TGFβ1, FN, Col Ⅳ in rosiglitazone＋ox-LDL group were also Rosiglitazone inhibited RMC proliferation, secretion of TGFβ1 antisirrhosis effect. ower than those in ox-LDL group （P〈0.05 or 0.01）. Conclusion and extracelluar matrix, which may be associated with its antisirrhosis effect.

关 键 词：罗格列酮 氧化低密度脂蛋白 系膜细胞 细胞外基质 

分 类 号：R692.6[医药卫生—泌尿科学] R587.1[医药卫生—外科学]