高糖对人近端肾小管上皮细胞TGF-β1、PAI-1基因表达的影响  

Synthesis of TGF-β1 and PAI-1 in human proximal tubular epithelial cells cultured with high glucose concentration

作　　者：刘芳[1] 陈烁苹[1] 郑景晨[1] 

机构地区：[1]温州医学院附属第一医院内分泌科,浙江温州325000

出　　处：《浙江医学》2009年第9期1248-1250,共3页Zhejiang Medical Journal

摘　　要：目的观察高糖（HG）对人近端肾小管上皮细胞（HKC）分泌转化生长因子β1（TGF-β1）和纤溶酶原激活物抑制因子1（PAI-1）mRNA表达水平的影响。方法采用数字表法随机分为低糖组（5.5mmol／L，LG组）和HG组（25mmol／L），分别培养于HKC中，于培养0、24和48h时收获细胞，并采用RT-PCR法检测两组HKC分泌TGF-β1和PAI-1 mRNA的表达水平。结果HG组培养24h和48h时的HKC分泌TGF-β1和PAI-1 mRNA表达水平均较LG组明显升高（均P〈0.01）。且两组培养48h时的上述指标均较24h时明显升高（均P〈0.O01）；经Spearman相关分析显示，HKC分泌PAI-1和TGF-β1 mRNA的表达水平之间存在正相关性（r=0.802,P〈0.01）。结论HG可刺激HKC分泌TGF-β1和PAI-1 mRNA表达水平的增高。Objective To investigate the effect of high glucose concentration on synthesis of TGF-β1 and PAI-1 mRNA in cultured human proximal tubular epithelial cells. Methods Human proximal tubular epithelial cells cultured with high glucose （25mmol/L, HG）or low glucose （5mmol/L, LG）concentrations,the expression of TGF-β1 and PAI-1 was assessed by RT-PCR. Results TGF-β1 and PAI-1 mRNA levels were significantly increased in HG groups compared with LG groups. Conclusion High glucose can significantly up-regulate TGF-β1 and PAI-1 gene expression in cultured human proximal tubular epithelial cells.

关 键 词：糖尿病肾病 转化生长因子Β1 纤溶酶原激活物抑制因子1 

分 类 号：R692[医药卫生—泌尿科学] R575.2[医药卫生—外科学]