宣肺通腑化瘀法对肺间质纤维化小鼠羟脯氨酸和转化生长因子-β1含量的影响  

Effect of dispersing lung-qi combined with purgation and eliminating stasis method （宣肺通腑化瘀法） on contents of hydroxyproline and transforming growth factor-β1 in mice with pulmonary fibrosis

作　　者：胡静[1] 郭永洁[1] 刘旭凌[2] 

机构地区：[1]上海中医药大学基础医学院,上海201203 [2]上海中医药大学研究生部,上海201203

出　　处：《中国中西医结合急救杂志》2009年第4期234-237,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：上海高校选拔培养优秀青年教师科研专项基金资助项目（szy05003）;辽宁省自然科学基金资助项目（2001101029）

摘　　要：目的探讨宣肺通腑化瘀法防治肺问质纤维化（PF）的作用及其机制。方法将28只ICR小鼠随机分为对照组、模型组、宣肺通腑化瘀法（中药）组、泼尼松组4组，每组7只。经鼻滴入博莱霉素建立小鼠PF模型。中药组和泼尼松组于制模8h内开始分别灌胃中药组方15．86g／kg或泼尼松1．3mg／kg，每日1次，连用28d；对照组及模型组给予等量生理盐水。于制模28d取小鼠肺组织观察病理学改变及纤维化程度；用酶联免疫吸附法测定支气管肺泡灌洗液（BALF）中转化生长因子-β1（TGF-β1）含量；用样本碱水解法检测肺组织中羟脯氨酸（HYP）含量。结果模型组小鼠肺泡炎症及纤维化程度均明显重于对照组；肺组织中HYP含量及BALF中TGF-β1含量均较对照组显著升高（P均〈0．01）。中药组和泼尼松组炎症及纤维化程度明显改善，HYP及TGF-β1含量均明显低于模型组（P均〈0．01），且中药组TGF-β1降低程度更为显著（P〈0．05）。结论宣肺通腑化瘀法能明显减轻博来霉素致小鼠PF程度，降低肺组织中HYP含量；其机制可能是通过抑制TGF-β1蛋白表达，减少胶原蛋白产生，使PF病变减轻。Objective To explore the effect and mechanism of dispersing lung-qi combined with purgation and eliminating stasis method （宣肺通腑化瘀法） on prevention and treatment of pulmonary fibrosis （PF）. Methods Twenty-eight ICR mice were randomly divided into four groups： control, model, dispersing lung-qi combined with purgation and eliminating stasis [traditional Chinese medicine （TCM）] and prednisolone groups （n= 7, each）. The PF model of mouse was established by bleomyein （BLM） nasal instillation. In 8 hours after the model establishment, the mice were respectively treated with intra-gastric infusion of 15.86 g/kg herb decoction in TCM group and 1.3 mg/kg dehydrocortisone in prednisolone group, once a day for 28 days ; while in the control and model groups, an equivalent amount of normal saline was given by gavage once daily. On the 28th day after the model establishment, the lung tissues were taken from the mice for histopathology study to observe the degree of airsacculitis and fibrosis. The transforming growth factor-β1 （TGF-β1） content of the bronchoalveolar lavage fluid （BALF） was tested by enzyme linked immunosorbent assay （ELISA）, and the hydroxyproline （HYP） level in lung tissues was measured by alkaline hydrolysis. Results The degrees of airsacculitis and fibrosis in model group were obviously higher than those in the control group. Compared with control group, the HYP content in lung tissues and the level of TGF-β1 in BALF were increased significantly in model group （all P〈0.01）. In TCM and prednisolone groups, the pathological degrees of airsaeculitis and fibrosis were improved obviously; the contents of HYP and TGF-β1 were lower than those in the model group （all P〈0. 01）, and the content of TGF-β1 was decreased more evidently in TCM group than that in the prednisolone group （P〈0.05）. Conclusion In the mouse model of PF induced by BLM, the TCM therapy, method of dispersing lung-qi combined with purgation and eliminating stasis, can reduce

关 键 词：肺间质纤维化 转化生长因子-Β1 羟脯氨酸 中药疗法 宣肺通腑化瘀法 

分 类 号：R285.5[医药卫生—中药学] R256.1[医药卫生—中医学]