芹菜素对大鼠急性脑缺血损伤的干预作用及机制研究  

Study on interventional effect of apigenin on acute cerebral ischemia injury in rats

作　　者：刘婵[1] 涂丰霞[1] 陈翔[1] 林晓燕[1] 张泷[1] 梁艳苓[1] 

机构地区：[1]温州医学院附属第二医院暨育英儿童医院康复中心和脑科中心,浙江温州325000

出　　处：《中国中西医结合急救杂志》2009年第4期238-241,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：浙江省教育厅项目资助（20020448）;浙江省温州市科技局国际合作项目（H20070034）

摘　　要：目的探讨芹菜素在大鼠急性局灶性脑缺血／再灌注（I／R）损伤中的抗炎作用及意义。方法将SD大鼠随机分为假手术组、模型组和芹菜素组，后两组按再灌注时间分为0．5h组和4h组，每组8只。采用改良大脑中动脉线栓法建立急性局灶性大鼠脑I／R模型；分别用干湿法和甲酰胺浸泡法定量测定各组大鼠脑组织含水量及伊文思蓝（EB）含量，用酶联免疫吸附法（ELISA）和比色法测定脑组织肿瘤坏死因子-α（TNF—α）、白细胞介素-1β（IL-1β）含量以及诱生型一氧化氮合酶（iNOS）活性。结果与假手术组比较，模型组大鼠脑组织含水量、EB含量及TNF—α、IL-1β含量、iNOS活性均显著升高，并与时间呈正相关（P〈0．05或P〈0．01）。芹菜素组各时间点脑组织含水量、EB含量及TNF-α、IL-1β含量、iNOS活性均较模型组显著下降（P〈0．05或P〈0．01）。结论芹菜素对急性局灶性脑I／R损伤的保护作用可能与抑制TNF—α启动的炎症反应有关。Objective To investigate the anti-inflammatory effects of apigenin on rats with acute transient focal cerebral ischemia/reperfusion （I/R） injury. Methods Sprague-Dawley （SD） rats were randomly divided into three groups ： sham-operated, model and apigenin-treated groups ; each of the latter two groups was subdivided into 0. 5 hour-reperfusion and 4 hour-reperfusion groups （each, n= 8）. The model of acute transient focal cerebral I/R was established by a modified method of creating cerebral artery thrombosis, inserting a nylon thread into the middle cerebral artery to let it stay there for 2 hours and then withdraw it from the artery. In these groups, brain water content and Evans blue （EB） content were measured quantitatively with wet and dry weight method and formamide immersion method respectively. The contents of tumor necrosis factor-α（TNF-α）, interleukin-1β （IL-1β） and activity of inducible nitric oxide synthase （iNOS） of the brain in these rats were essayed by enzyme linked immunosorbent assay （ELISA） and chromometry respectively. Results The brain contents of water, EB, TNF-α, IL-1β and the activity of iNOS in the model group were significantly higher than those in the sham-operated group; in the brain of model group, there were close positive correlations between the contents of TNF-α, IL-1β, the activity of iNOS and the contents of brain water or EB respectively （P〈0.05 or P〈0. 01）. The brain contents of water, EB, TNF-α, IL-1β and the activity of iNOS in apigenin-treated group were obviously lower than those in the model group at each time point （P 〈 0. 05 or P 〈 0.01）. Conclusion Apigenin may play an important neuroprotective role in rats with acute transient focal cerebral I/R injury ; the mechanism is probably related to the inhibition of TNF-α initiating inflammatory reaction.

关 键 词：芹菜素 缺血/再灌注损伤 脑 肿瘤坏死因子-α 白细胞介素-1Β 诱生型一氧化氮合酶 

分 类 号：R965[医药卫生—药理学] R743.3[医药卫生—药学]