糖的基质辅助激光解吸/电离质谱(MALDI-MS)研究  

Matrixassisted Laser Desorption/Ionization Mass Spectral Study of Saccharides

作　　者：郝春雁[1,2] 马秀俐[1,2] 刘志强[1,2] 季怡萍[1,2] 刘淑莹[1,2] 刘长城 孙允秀[1,2] 刘举正 

机构地区：[1]中国科学院长春应用化学研究所 [2]吉林大学化学系

出　　处：《高等学校化学学报》1998年第7期1090-1094,共5页Chemical Journal of Chinese Universities

基　　金：国家中医药管理局资助

摘　　要：通过糖类化合物３种常用基质ＭＡＬＤＩ－ＭＳ分析效果的比较以及寡糖和多糖正、负离子ＭＡＬＤＩ－ＭＳ谱的对比，找到了适合糖分析的基质２，５－ＤＨＢ，探讨了糖类化合物激光解吸／电离条件下形成离子的过程，指出了Ｎａ＋、Ｋ＋离子在寡糖分子量测定中的重要作用，借助柱层析分离手段，成功地测出了分子量大于１００００的葡聚糖的分子量．Matrixassisted laser desorption/ionization (MALDI) mass spectra of saccharides with three common matrixes were studied here. It is shown that Na + and K + play important roles in the determination of oligasaccharide molecule weight and the molecular weights(MW) of glucans(dextran) with MW more than 10 000 is determined successfully with the help of column chromatography. By comparing the effects of three matrixes in saccharide analysis and comparing positive ion and negative ion matrixassisted laser desorption/ionization mass spectra of oligo and polysaccharides, the most suitable matrix for saccharide analysis, 2,5 dihydroxylbezonic acid (DHB), is selected and the ion formation processes of saccharides under matrix assisted laser adsorption/ionization condition are clarified.

关 键 词：MALDI-MS 寡糖 多糖 分子量测定 葡聚糖 糖类 

分 类 号：Q530.3[生物学—生物化学]