A covalent modified hydrophilic capillary for enhanced capillary electrophoresis of biopolymers  

作　　者：Lian Guo Shan Xue Yu Yin Mao Wei Xiao Hui Zheng Jian Bin Zheng 

机构地区：[1]College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China [2]Institute of Analytical Science, Northwest University, Xi'an 710069, China

出　　处：《Chinese Chemical Letters》2009年第9期1123-1126,共4页中国化学快报（英文版）

基　　金：National Natural Science Foundation of China(Nos.20875075 and 20875074)

摘　　要：8-Gluconolactone was covalently coupled to aminopropyl derivatized capillary, which created hydrophilic brushes on the inner wall of the capillary. The coated capillary was shown to generate a stable electroosmotic flow （EOF） in the investigated pH range of 2.0-9.0 and to suppress effectively the adsorption of proteins. And it enabled separation of some biopolymer mixtures including basic proteins, DNA and tryptic digested bovine serum albumin （BSA） within 15 rain with efficiencies up to 450,000 plates/m. The intra- and inter-day reproducibility of the coating referring to the retention times of proteins were satisfactory with mean relative standard deviations （R.S.D.） of 0.8 and 1.7%, respectively. 2009 Yin Mao Wei. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved.8-Gluconolactone was covalently coupled to aminopropyl derivatized capillary, which created hydrophilic brushes on the inner wall of the capillary. The coated capillary was shown to generate a stable electroosmotic flow （EOF） in the investigated pH range of 2.0-9.0 and to suppress effectively the adsorption of proteins. And it enabled separation of some biopolymer mixtures including basic proteins, DNA and tryptic digested bovine serum albumin （BSA） within 15 rain with efficiencies up to 450,000 plates/m. The intra- and inter-day reproducibility of the coating referring to the retention times of proteins were satisfactory with mean relative standard deviations （R.S.D.） of 0.8 and 1.7%, respectively. 2009 Yin Mao Wei. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved.

关 键 词：Capillary electrophoresis Capillary coating 8-Gluconolactone Protein separation 

分 类 号：O657.8[理学—分析化学] TQ028.8[理学—化学]