脑脉通对老龄大鼠脑缺血／再灌注海马内源性神经干细胞增殖分化的影响  

Effect of Naomaitong （脑脉通） on proliferation and differentiation of hippocampus endogenous neural stem cell in aged rats with cerebral isehemia/reperfusion

作　　者：高剑峰[1,2] 朱长连[1] 李建生[2] 

机构地区：[1]郑州大学第三附属医院,河南郑州450052 [2]河南中医学院,河南郑州450008

出　　处：《中国中西医结合急救杂志》2009年第1期26-29,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金项目（30371812）

摘　　要：目的从海马齿状回（DG）细胞增殖和分化的变化揭示脑脉通抗老年脑缺血／再灌注（I／R）损伤的保护机制及其量-效关系。方法采用大脑中动脉闭塞（MCAO）方法造成老龄大鼠脑缺血3h、再灌注6d模型，观察脑脉通大、中、小剂量（分别为26．0、13．0、6．5g·kg^-1·d^-1）对脑I／R大鼠神经症状评分、脑组织含水量、病理学变化、DG细胞增殖、分化及其变化的影响。以尼莫地平作为阳性对照组。结果模型组神经症状评分、脑组织含水量及5-溴-2-脱氧尿嘧啶核苷（BrdU）阳性细胞、BrdU／神经元核蛋白（NeuN）双标阳性细胞、BrdU／胶质原纤维酸性蛋白（GFAP）双标阳性细胞数均高于假手术组（P均〈0．01）。脑脉通各组、尼莫地平组神经症状评分、脑组织含水量及Brdu／GFAP双标阳性细胞数均低于模型组，其中以脑脉通中剂量组最低，分别为（0．77±0．92）分、（78．17±1．99）％、（33．71±3．01）个／mm^2；BrdU阳性细胞、BrdU／NeuN双标阳性细胞数明显高于模型组（P〈0．05或P〈0．01），其中以脑脉通中剂量组最高，分别为（187．03±7．15）个／mm^2，（42．14±3．09）个／mm^2。结论脑缺血可激活大鼠DG神经干细胞增殖；脑脉通拮抗脑I／R损伤机制与其促进DG区神经干细胞增殖和分化有关，且以脑脉通中剂量效果最显著。Objective To expose the protective effect of Naomaitong （脑脉通） on senile cerebral ischemia/reperfusion （I/R） injury from the changes of cell proliferation and differentiation at dentate gyrus （DG） of hippocampus and to approach its mechanism and dose-effect relationship. Methods Middle cerebral artery occlusion （MCAO） was applied to establish aged rat model of cerebral ischemia for 3 hours and reperfusion for 6 days. Large, medium and low dose of Naomaitong （respectively 26.0, 13.0 and 6.5 g · kg^-1 · d^-1） were used to observe the effects on changes of nervous dysfunction score, the water content of cerebral constitution, pathological changes and the stem cell proliferation and differentiation in DG in aged rats with cerebral I/R injury. The aged rats treated with nimodipine was as control group. Results The nervous dysfunction score, the water content of cerebral constitution, 5-bromo-2-deoxyuridine （BrdU） positive cells, BrdU/neuron neucleoprotein （NeuN） positive cells, BrdU/gliofibrillary acid protein （GFAP） positive cells in model group were higher than those in the sham operation group （all P〈0. 01）. The nervous dysfunction score, the water content of cerebral constitution, BrdU/GFAP positive cells in various Naomaitong groups and nimodipine group were lower than those in the model group, and the medium dose of Naomaitong group was the lowest [0. 77±0. 92, （78. 17±1.99）%, （33.71±3.01） cells/mm^2, P〈0.05 or P〈0. 01）; the BrdU positive cell and BrdU/NeuN positive cell numbers were significantly higher than those in the model group, especially the medium dose group, being the highest [（187.03 ± 7.15） cells/mm^2, （42.14±3. 09） eells/mm^2, P〈0. 05 or P〈0. 01]. The nervous dysfunction score, the BrdU positive cells, BrdU/NeuN positive cells were decreased compared with those of the nimodipine group. Conclusion The proliferation of neural stem cells at DG in aged rats is activated after cerebral ischemia; the protective effect of Naomaiton

关 键 词：脑脉通 老年 缺血/再灌注损伤 脑 神经干细胞 增殖 分化 

分 类 号：R285.5[医药卫生—中药学] Q254[医药卫生—中医学]